Detection of wheat allergens using 2D Western blot and mass spectrometry

ConclusionsThe present study allowed the identification of different protein areas specific to these studied groups. The protein spots of interest were identified by UHPLC-MS/MS. It has been possible to establish a link between a specific symptomatology and the newly identified responsible allergens.
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 18 November 2019Source: The Journal of Allergy and Clinical Immunology: In PracticeAuthor(s): Puja Sood Rajani, Hayley Martin, Marion Groetch, Kirsi M. JärvinenAbstractBreastfeeding is currently recommended as the optimal source of nutrition to infants. However, there are several studies that have shown clinical IgE and non-IgE-mediated reactions to foods in exclusively breastfeeding infants, specifically to cow’s milk, egg, peanut, and fish. Literature suggests that antigenic food proteins present in human milk can be found in substantial enough amounts to elicit clinical reac...
Source: The Journal of Allergy and Clinical Immunology: In Practice - Category: Allergy & Immunology Source Type: research
CONCLUSION: PFS can be seen in children who are followed up for pollen-induced AR. The symptoms of PFS are usually mild and transient. However, comprehensive evaluation of patients is important since serious systemic reactions such as anaphylaxis can also be observed. PMID: 31601505 [PubMed - as supplied by publisher]
Source: Allergologia et Immunopathologia - Category: Allergy & Immunology Authors: Tags: Allergol Immunopathol (Madr) Source Type: research
In this study, we investigated the clinical manifestations and risk factors for anaphylaxis in PFAS in Korean patients with pollinosis. MATERIALS AND METHODS: Data were obtained from a nationwide cross-sectional study that previously reported on PFAS in Korean patients with pollinosis. Data from 273 patients with PFAS were collected, including demographics, list of culprit fruits and vegetables, and clinical manifestations of food allergy. We analyzed 27 anaphylaxis patients and compared them with patients with PFAS with oropharyngeal symptoms only (n=130). RESULTS: The most common cause of anaphylaxis in PFAS wa...
Source: Yonsei Medical Journal - Category: Universities & Medical Training Authors: Tags: Yonsei Med J Source Type: research
CONCLUSION: Despite their widespread use, triazoles seem to induce rare cutaneous hypersensitivity reactions, but the pathomechanisms, risk factors, diagnostic and management strategies, including skin tests and challenge tests, are little known and poorly investigated. PMID: 31538908 [PubMed - as supplied by publisher]
Source: Recent Patents on Inflammation and Allergy Drug Discovery - Category: Allergy & Immunology Tags: Recent Pat Inflamm Allergy Drug Discov Source Type: research
Although atopic dermatitis (AD) has been associated with increased risk of food allergy (FA), this association is not fully understood, and the predictors of severe reactions to foods are not clear. In a review of 18 population-controlled studies, the rate of food sensitization was up to 6 times higher in patients with AD vs healthy controls.1 A recent study identified a potential physiologic mechanism by demonstrating increased skin barrier dysfunction, measured by increased transepidermal water loss and reduced skin filaggrin levels, in patients with AD and FA.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Tags: Letters Source Type: research
PMID: 31520770 [PubMed - as supplied by publisher]
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Tags: Ann Allergy Asthma Immunol Source Type: research
Although atopic dermatitis (AD) has been associated with increased risk of food allergy (FA), this association is not fully understood and the predictors of severe FA are not clear. In a review of 18 population-controlled studies, the rate of food sensitization was up to 6 times higher in patients with AD versus normal controls.1 A recent study identified a potential physiologic mechanism by demonstrating increased skin barrier dysfunction, measured by increased transepidermal water loss and reduced skin filaggrin levels, in patients with AD and FA.
Source: Annals of Allergy, Asthma and Immunology - Category: Allergy & Immunology Authors: Tags: Letters Source Type: research
This article is protected by copyright. All rights reserved. PMID: 31209867 [PubMed - as supplied by publisher]
Source: The British Journal of Dermatology - Category: Dermatology Authors: Tags: Br J Dermatol Source Type: research
We describe the case of a young boy affected by coeliac disease who, after an occasional unexpected ingestion of gluten, experienced a complete anaphylactic reaction characterized by urticarial, labial angioedema, wheezing, and hypotension. To better investigate the state of allergic sensitization to wheat in our patient, we then performed the component resolved diagnosis, which showed Tri a19 2 kU/l and Tri a14 0.3 kU/l. These results demonstrated the association of IgE-mediated allergy to wheat and coeliac disease. The natural course of specific IgE in allergic patients who are on a food-free diet needs fur...
Source: European Journal of Gastroenterology and Hepatology - Category: Gastroenterology Tags: Case Report Source Type: research
Authors: Aggarwal A, Balogun R, Carr TF, Desai AP, Jie T, Pan JJ Abstract 31 years old female with a history of contact dermatitis, eczema, allergic rhinitis, pernicious anemia, alopecia areata and latent tuberculosis was treated concurrently with methotrexate along with isoniazid and pyridoxine. Five months into the therapy she developed acute onset jaundice progressing into fulminant liver failure with altered mentation and worsening liver function tests. Extensive workup including serological and histopathological evaluation revealed drug-induced liver injury as the etiology of her liver failure and she underwen...
Source: Annals of Hepatology - Category: Gastroenterology Tags: Ann Hepatol Source Type: research
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