No protection in chickens immunized by the oral or intra-muscular immunization route with Ascaridia galli soluble antigen.

The objective of this study was to evaluate the induction of protective immune responses to A. galli soluble antigen by different immunization routes. Chickens were immunized with a crude extract of A. galli via an oral or intra-muscular route using cholera toxin B subunit as adjuvant and subsequently challenged with A. galli. Only chickens immunized via the intra-muscular route developed a specific A. galli antibody response. Frequencies of γδ T cells in spleen were higher 7 days after the first immunization in both groups but only significantly so in the intra-muscularly immunized group. In addition, systemic immunization had an effect on both Th1 and Th2 cytokines in caecal tonsils and Meckel's diverticulum. Thus both humoral and cellular immune responses are inducible by soluble A. galli antigen, but in this study no protection against the parasite was achieved.
PMID: 23718808 [PubMed - in process]

Source: Avian Pathology - June 1, 2013 Category: Pathology Authors: Andersen JP, Norup LR, Dalgaard TS, Rothwell L, Kaiser P, Permin A, Schou TW, Fink DR, Jungersen G, Sørensen P, Juul-Madsen HR Tags: Avian Pathol Source Type: research