Characterization of DNA cleavage produced by seminal plasma using leukocytes as a cell target.

In conclusion, capacity of seminal plasma for producing DNA cleavage represents a solid contribution to expand the analysis of the standard seminal profile and could constitute a putative diagnostic tool for evaluating male infertility. Abbreviations: ALS: alkali labile sites; ART: Assisted Reproduction Technologies; DBD-FISH: DNA Breakage Detection-Fluorescence In Situ Hybridization; DNA: deoxyribonucleic acid; DSBs-DNA: double-strand DNA; FITC: Fluorescein IsoThioCyanate; GEDA: Gravity Enforced Diffusion Assays; PBS: phosphate-buffered saline; ROS: Reactive Oxigen Species; SSBs-DNA: single-strand DNA; SSC: saline-sodium citrate. PMID: 31539284 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/31539284?dopt=Abstract

Source: Systems Biology in Reproductive Medicine - September 19, 2019 Category: Reproduction Medicine Authors: Cortés-Gutiérrez EI, De La Vega CG, Bartolomé-Nebreda J, Gosálvez J Tags: Syst Biol Reprod Med Source Type: research