Development and validation of a real-time multiplex PCR assay for the detection of dermatophytes and Fusarium spp.

Conclusion: The multiplex qPCR assays were reliable for the diagnosis of onychomycosis, with shorter turn-around time as compared to culture method. This aids in the planning of treatment strategies to achieve optimal therapeutic outcome. PMID: 31526456 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research

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AbstractPurpose of ReviewAs both onychomycosis and HIV/AIDS are frequently encountered, we aimed to make a review of the existent information to this moment, including classification, evaluation and treatment.Recent FindingsThe new description of dermatophytes will surely originate changes in the classification and probably in the therapeutic approach. Also, we must be watchful for the modifications that may develop in this pathology with the newer antirretrovirals which are in constant evolution and could modify the patients ’ response to dermatophytosis.SummaryAt this moment, we know that onychomycosis is very comm...
Source: Current Fungal Infection Reports - Category: Infectious Diseases Source Type: research
This study investigated the in vitro hydrolytic enzymes production, adhesion and biofilm formation capacity of Candida parapsilosis complex species and Kodamaea ohmeri isolates from onychomycoses of HIV/AIDS patients and also established the antifungal sensitivity profiles of these isolates. Onychomycosis in HIV/AIDS patients showed a high prevalence of emerging yeasts, among which C. parapsilosis complex species and K. ohmeri were the most frequent. Three C. parapsilosis sensu stricto and two C. orthopsilosis isolates were resistant to amphotericin B and 83% of isolates were resistant to terbinafine. All three different s...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
AbstractPurpose of ReviewThis review summarizes the most relevant information on onychomycosis in patients with immunosuppressive disorders and also compares the distinctive characteristics and approach with the general population.Recent FindingsOnychomycosis in immunocompromised patients has a similar clinical presentation to the one in the general population, yet there are still differences. For instance, the white variety also known as mycotic leukonychia is characteristic among the group, being more common among patients with HIV/AIDS. Proximal subungual onychomycosis (PSO) and white superficial onychomycosis (WSO) hav...
Source: Current Fungal Infection Reports - Category: Infectious Diseases Source Type: research
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