Chitosan-based hydrogel wound dressings with electrochemically incorporated silver nanoparticles – in vitro study

Publication date: Available online 18 September 2019Source: European Polymer JournalAuthor(s): Katarina Nešović, Ana Janković, Tamara Radetić, Maja Vukašinović-Sekulić, Vesna Kojić, Ljiljana Živković, Aleksandra Perić-Grujić, Kyong Yop Rhee, Vesna Mišković-StankovićAbstractIn this work, we provide a facile path for the preparation of wound dressing materials with improved characteristics based on biocompatible chitosan and poly(vinyl alcohol) hydrogels with embedded silver nanoparticles (AgNPs) as a potent antimicrobial agent. The hydrogels were prepared via freezing-thawing method, whereas AgNPs were synthesized via an in situ electrochemical route. The excellent physicochemical properties of the obtained nanocomposite hydrogels were validated using different spectroscopic and microscopic techniques. The formation of sub-10 nm AgNPs was confirmed by UV-visible spectroscopy, dynamic light scattering and transmission electron microscopy. Swelling and silver release profiles were validated using several diffusion models. The hydrogels were proven to be non-cytotoxic using MTT test and possessed powerful antibacterial activity against Staphylococcus aureus and Escherichia coli, as determined by test in suspension, which affirmed their feasibility for wound dressing applications.Graphical abstract
Source: European Polymer Journal - Category: Chemistry Source Type: research