Structural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4 [Biochemistry]

Prokaryotes possess CRISPR-Cas systems to exclude parasitic predators, such as phages and mobile genetic elements (MGEs). These predators, in turn, encode anti-CRISPR (Acr) proteins to evade the CRISPR-Cas immunity. Recently, AcrVA4, an Acr protein inhibiting the CRISPR-Cas12a system, was shown to diminish Lachnospiraceae bacterium Cas12a (LbCas12a)-mediated genome editing in human...

Source: Proceedings of the National Academy of Sciences - September 16, 2019 Category: Science Authors: Ruchao Peng, Zhiteng Li, Ying Xu, Shaoshuai He, Qi Peng, Lian-ao Wu, Ying Wu, Jianxun Qi, Peiyi Wang, Yi Shi, George F. Gao Tags: PNAS Plus Source Type: research