Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research

Publication date: Available online 16 September 2019Source: Stem Cell ResearchAuthor(s): Erica C. Pandolfi, Ernesto J. Rojas, Enrique Sosa, Joanna J. Gell, Timothy J. Hunt, Sierra Goldsmith, Yuting Fan, Sherman J. Silber, Amander T. ClarkAbstractWe generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDF) (MZT04) generated from a skin biopsy donated from a fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility and infertility. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using teratomas. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility and infertility.
Source: Stem Cell Research - Category: Stem Cells Source Type: research

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CONCLUSIONS: Apoptosis is an important clinical target to protect NSCs during brain tumor radiotherapy using carbon-ion radiation as well as conventional X-rays. PMID: 31633435 [PubMed - as supplied by publisher]
Source: International Journal of Radiation Biology - Category: Radiology Tags: Int J Radiat Biol Source Type: research
Rancho Cordova-based Cesca Therapeutics Inc. says it's launching a joint venture to create a blood bank-style service for immune cells. Cesca (NASDAQ: KOOL) announced its joint venture with Irvine-based HealthBanks Biotech USA Inc. on Tuesday. Both companies are affiliated with Boyalife Group Inc., a Chinese group of stem cell companies. Boyalife bought a 70% stake in Cesca in 2016, and HealthBanks is a subsidiary. The joint vent ure plans to create a facility that would be “among the first immune…
Source: bizjournals.com Health Care:Biotechnology headlines - Category: Biotechnology Authors: Source Type: news
The transcription factor B cell CLL/lymphoma 11B (BCL11B) is indispensable for T lineage development of lymphoid progenitors. Here, we show that chimeric antigen receptor (CAR) expression during early phases of ex vivo generation of lymphoid progenitors suppressed BCL11B, leading to suppression of T cell–associated gene expression and acquisition of NK cell–like properties. Upon adoptive transfer into hematopoietic stem cell transplant recipients, CAR-expressing lymphoid progenitors differentiated into CAR-induced killer (CARiK) cells that mediated potent antigen-directed antileukemic activity even across MHC b...
Source: Journal of Clinical Investigation - Category: Biomedical Science Authors: Source Type: research
UCLA researchers have made new inroads into understanding germ cell tumors, a diverse and rare group of cancers that begin in germ cells — the cells that develop into sperm and eggs. The researchers developed a protocol to recreate germ cell tumor cells from stem cells and used the new model to study the genetics of the cancer.Their findings could point the way toward new drugs to treat germ cell tumors, which account for around 3 percent of all cases of childhood and adolescent cancer.The study, published in Stem Cell Research, was led by Amander Clark, a UCLA professor of molecular cell and developmental biology an...
Source: UCLA Newsroom: Health Sciences - Category: Universities & Medical Training Source Type: news
Publication date: March 2018 Source:Stem Cell Research, Volume 27 Author(s): Fang Fang, Zili Li, Qian Zhao, Zhen Ye, Kai Zhao, Xiuli Gu, Shiliang Shen, Renee A. Reijo Pera, Chengliang Xiong Human fibroblasts were isolated from foreskin of a clinically diagnosed 40-year old patient with idiopathic infertility. The fibroblasts were reprogrammed with the Yamanaka KOSM transcriptional factors using the retroviral vectors. The obtained induced pluripotent stem cell (iPSC) line showed pluripotency verified by the expression of pluripotency markers, NANOG, SOX2, OCT4, TRA-1-60, and SSEA-4. And the iPSC line was demonstrated to h...
Source: Stem Cell Research - Category: Stem Cells Source Type: research
We present a patient with infertility for PCOS. She was overweight and her medical history included IR, right-sided ovarian mucinous cystadenomas, and left-sided teratoma. Although she had been treated with ovarian surgery, clomiphene citrate and gonadotropins, weight loss and metformin, which have been effective for dominant follicle development, she still failed to conceive. Then EX was initiated to intervene for 2 months. EX treatment was successful to improve IR; after that the infertile woman with PCOS became pregnant. EX improves IR and reproduction capacity in PCOS patients, reducing insulin level and ameliorating e...
Source: Gynecologic and Obstetric Investigation - Category: OBGYN Source Type: research
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Source: Gynecologic Oncology Reports - Category: OBGYN Source Type: research
Authors: Choussein S, Hariton E, Bortoletto P, Gargiulo A Abstract Even in the context of the expansion of assisted reproductive technologies (ART), reproductive surgery continues to fulfill its deserved place in the arsenal of fertility treatments. Rather than being competitive, these two avenues of treatment can optimize fertility care when utilized as an adjunct to one another. Surgical indications and techniques in modern reproductive surgery continue to evolve based on new information about the effects of pelvic pathology on infertility and new technology. This review aims to disentangle some of the common cli...
Source: Minerva Ginecologica - Category: OBGYN Tags: Minerva Ginecol Source Type: research
CONCLUSIONS: KS patient-derived iPS cells that could differentiate into cardiomyocyte-like cells were established. PMID: 26709348 [PubMed - as supplied by publisher]
Source: Reproductive Biology - Category: Reproduction Medicine Authors: Tags: Reprod Med Biol Source Type: research
Conclusions KS patient-derived iPS cells that could differentiate into cardiomyocyte-like cells were established.
Source: Reproductive Medicine and Biology - Category: OBGYN Source Type: research
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