Comparative Performance of High-Risk Human Papillomavirus RNA and DNA In Situ Hybridization on College of American Pathologists Proficiency Tests.

CONCLUSIONS.—: An assessment of CAP HR-HPV proficiency test performance indicates that RNA ISH shows significantly higher accuracy than DNA ISH owing to higher analytical sensitivity of RNA ISH in tumors with low (1-2 copies per cell) to intermediate (50-100 copies per cell) HR-HPV viral copy numbers. These data support the use of RNA over DNA ISH in clinical laboratories that perform HR-HPV testing as part of their testing algorithms.
PMID: 31483999 [PubMed - as supplied by publisher]

Source: Archives of Pathology and Laboratory Medicine - September 3, 2019 Category: Laboratory Medicine Authors: Keung ES, Souers RJ, Bridge JA, Faquin WC, Graham RP, Hameed MR, Lewis JS, Merker JD, Vasalos P, Moncur JT Tags: Arch Pathol Lab Med Source Type: research