Antiradical properties of chemo drug, carboplatin, in cooperation with ZnO nanoparticles under UV irradiation in putative model of cancer cells

Publication date: Available online 4 September 2019Source: Biocybernetics and Biomedical EngineeringAuthor(s): Suttirak Pairoj, Pattareeya Damrongsak, Badin Damrongsak, Natini Jinawath, Rossukon Kaewkhaw, Tanaporn Leelawattananon, Chinnapat Ruttanasirawit, Kitsakorn LocharoenratAbstractThe main objective of this study was to assess the antiradical properties of zinc oxide (ZnO) nanoparticles upon exposure to ultraviolet radiation with carboplatin, an anti-proliferative drug used in the treatment of retinoblastoma. For the purpose of this study, the decomposition of 2,2(diphenyl-1-picryhydrazyl) radical (DPPH*) was used to assess the free radical capacity of antioxidants and was followed by MTT measurements. To test the antiradical capacity, the effective concentration, antiradical power, stoichiometry, and number of reduced DPPH* were investigated. DPPH* has a peak absorbance at a wavelength of 515 nm, which disappears upon the introduction of the antiradical agents. Four agents were reacted with DPPH* and represented the possible reaction kinetic categories. ZnO nanoparticles and carboplatin-loaded ZnO nanoparticles reacted more strongly with DPPH* and approached a saturation state at 420 min. The remaining two antiradical agents, ZnO nanoparticles under UV radiation and carboplatin-loaded ZnO nanoparticles under UV radiation, reacted a bit slower with DPPH* and approached a steady state at 1440 min. Among the different four antiradical agents, carboplatin-loaded ZnO n...
Source: Biocybernetics and Biomedical Engineering - Category: Biomedical Engineering Source Type: research