Molecular characterization and computational structure prediction of activin receptor type IIB in aseel and broiler chicken

Publication date: Available online 27 August 2019Source: Research in Veterinary ScienceAuthor(s): P. Guru Vishnu, T.K. Bhattacharya, Bharat Bhushan, Pushpendra Kumar, R.N. Chatterjee, Chandan Paswan, A. Rajendra Prasad, D. Divya, K. DushyanthAbstractThe present study was formulated to characterize and comprehend the molecular structural characteristics of ACTRIIB receptor in Aseel and control broiler (CB) populations. The full length coding sequence (1539 bp) of the receptor was amplified, cloned, sequenced and analyzed using bioinformatic tools. The physico chemical properties of protein and structural features like secondary structure, solvent accessibility and disorder regions were computed. The 3D structure was predicted by I-TASSER and evaluated by Ramachandran Plot and tools under Structural Analysis and Verification Server. The nucleotides differences between CB and Aseel were c. [156G > A; 210 T > C; 493C > T; c.520G > C; 665A > C; 686G > A; 937C > G; 1011A > C; 1130A > G; 1208 T > A; 1326 T > C; 1433 T > C]. The amino acid substitutions between CB and Aseel were p. [(Pro165Ser; Glu174Gln; Gln222Pro; Ser229Asn; His313Asp; Gln377Arg; Val403Asp; and Ile478Thr)]. While, the silent changes includes p. [(Lys53=; Glu71=; Leu337=; Asp442=)]. The molecular weight of mature protein was predicted to be 55.51 kDa and 57.80 kDa in Aseel and CB, respectively. The higher rank 3D model had a C-score of −1.60 in Aseel an...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research