Short communication: Gut microbial colonization of the mouse colon using faecal transfer was equally effective when comparing rectal inoculation and oral inoculation based on 16S rRNA sequencing
Publication date: Available online 17 September 2019Source: Research in Veterinary ScienceAuthor(s): Ditte Olsen Lützhøft, Lidia Sánchez-Alcoholado, Peter Tougaard, Caroline M. Junker Mentzel, Witold Kot, Dennis S. Nielsen, Axel Kornerup HansenAbstractIn the present study we hypothesized that a higher degree of gut microbiota (GM) transfer and colonization could be reached by rectal inoculation compared to oral inoculation, which is commonly used in mice studies for GM transfer. We treated C57BL/6NTac Specific Pathogen Free (SPF)-mice with antibiotics and subsequently we inoculated these with GM from don...
Source: Research in Veterinary Science - September 17, 2019 Category: Veterinary Research Source Type: research

Letter to the Editor
Publication date: Available online 13 September 2019Source: Research in Veterinary ScienceAuthor(s): (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - September 15, 2019 Category: Veterinary Research Source Type: research

The inhibitory effect of flavonoids and their gut-derived metabolites on the replication of Chlamydia abortus in the AH-1 ovine trophoblast cell line
Publication date: Available online 12 September 2019Source: Research in Veterinary ScienceAuthor(s): Laura del Rio, Jesús Salinas, Antonio J. Buendía, María-Teresa García-ConesaAbstractThe obligate intracellular bacterium Chlamydia abortus causes abortion and constitutes a worldwide threat for livestock. Plant-derived flavonoids have antimicrobial effects against veterinary and human pathogens and may be of help in the fight against C. abortus. The anti-infective efficacy against C. abortus of the flavonoids apigenin and naringenin, and of four of their derived metabolites was explored using an ...
Source: Research in Veterinary Science - September 13, 2019 Category: Veterinary Research Source Type: research

Effect of piperine and quercetin alone or in combination with marbofloxacin on CYP3A37 and MDR1 mRNA expression levels in broiler chickens
This study was carried out to investigate the effect of piperine and quercetin alone or in combination with marbofloxacin on CYP3A37 and MDR1 mRNA expression levels in liver and intestine of broiler chicken. After oral administration of piperine and quercetin for 3 consecutive days followed by with or without oral administration of marbofloxacin for 5 days, CYP3A37 and MDR1 mRNA expression levels were determined using quantitative real-time PCR. Total of 36 broiler chickens in seven individual groups were treated with different regimen and the mRNA expression levels at duodenum and liver were analyzed with apt statistica...
Source: Research in Veterinary Science - September 13, 2019 Category: Veterinary Research Source Type: research

Modelling for the inheritance of multiple births and fertility in endangered equids: Determining risk factors and genetic parameters in donkeys (Equus asinus)
The objective of this study was to examine the historical foaling records of Andalusian donkeys to estimate prevalence, risk factors, phenotypic and genetic parameters for multiple births, assessing the cumulative foal number born per animal, maximum foal number per birth and multiple birth number per animal. We designed a Bayesian General Animal Mixed Model with single records considering the ‘fixed’ effects of birth year, birth season, birth month, sex, farm, location, and husbandry system. Age was considered and included as a linear and quadratic covariate. Gibbs sampling reported heritability estimates rang...
Source: Research in Veterinary Science - September 13, 2019 Category: Veterinary Research Source Type: research

HoBi-like pestivirus infection in an outbreak of bovine respiratory disease
In this study we reported the detection of HoBi-like pestivirus in an outbreak of respiratory disease in calves from Brazil, seropositive for viruses of the bovine respiratory disease complex (BRDC). Thus, serum samples and nasal swabs were collected from calves up to one year old, presenting or not clinical signs of respiratory disease. Serum samples were submitted to virus neutralization test (VNT) for BVDV-1, BVDV-2, bovine herpesvirus-1 (BoHV-1), bovine respiratory syncytial virus (BRSV) and bovine parainfluenza-3 (BPIV-3). These samples were also tested for the presence of pestiviruses (BVDV-1, BVDV-2 and HoBi-like) a...
Source: Research in Veterinary Science - September 13, 2019 Category: Veterinary Research Source Type: research

Therapeutic applications of adipose-derived mesenchymal stem cells on acute liver injury in canines
In this study, canine adipose-derived mesenchymal stem cells (cADSCs) therapeutic potential was investigated in artificially induced acute liver injury model by CCl4 in canines. The primary cADSCs cells were cultured and then intravenously administered into the canine animal model. Six cross-breed dogs were divided into three groups including blank control group, CCl4 model group, CCl4 induced cADSCs transplantation group.The results showed that after intraperitoneal injection of CCl4 solution, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and Albumin (ALB) in peripheral blood of experiment...
Source: Research in Veterinary Science - September 12, 2019 Category: Veterinary Research Source Type: research

Trichostatin A and vorinostat promote adipogenic differentiation through H3K9 acetylation and dimethylation
Publication date: Available online 12 September 2019Source: Research in Veterinary ScienceAuthor(s): Xiao Wang, Zhimin Wang, Qing Wang, Hao Liang, Dongjun LiuAbstractTo explore the effect of epigenetic modification on the differentiation of goat adipose-derived stem cells in vitro, we used two common epigenetic modification inhibitors, trichostatin A and vorinostat, to treat cashmere goat adipose-derived stem cells and induce adipocyte differentiation. The results showed that trichostatin A and vorinostat changed the relative amounts of H3K9 acetylation and dimethylation in the upstream sequence of PPARG, increased peroxis...
Source: Research in Veterinary Science - September 12, 2019 Category: Veterinary Research Source Type: research

Reply to the Letter to the Editor of Dr. Baker
Publication date: Available online 10 September 2019Source: Research in Veterinary ScienceAuthor(s): A. Muñoz-Prieto, S. Martínez-Subiela, J.J. Cerón, A. Tvarijonaviciute (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - September 12, 2019 Category: Veterinary Research Source Type: research

Development of conventional and real time PCR assays for rapid species authentication of mammalian cell lines commonly used in veterinary diagnostic laboratories
Publication date: Available online 30 August 2019Source: Research in Veterinary ScienceAuthor(s): Amaresh Das, Lizhe Xu, Wei JiaAbstractMammalian cell lines are valuable tools in biomedical fields, with applications ranging from disease diagnosis to the production of biological reagents and vaccines. Here we report the development of new conventional (cPCR) and real time PCR (qPCR) assays for species identification of several mammalian kidney cell lines originated from swine, green monkey, hamster and bovine tissues that are extensively used in veterinary diagnostic laboratories. The PCR primers and probes were selected fr...
Source: Research in Veterinary Science - August 31, 2019 Category: Veterinary Research Source Type: research

Neutrophil-to-lymphocyte ratio, nucleated red blood cells and erythrocyte abnormalities in canine systemic inflammatory response syndrome
Publication date: Available online 28 August 2019Source: Research in Veterinary ScienceAuthor(s): A. Pierini, E. Gori, I. Lippi, G. Ceccherini, G. Lubas, V. MarchettiAbstractSystemic inflammatory response syndrome (SIRS) is the manifestation of the systemic response to an infectious or non-infectious disease. We evaluated the association between erythrocyte parameters, including nucleated red blood cells (NRBCs) and leukocyte ratios (NLR, neutrophil-to-lymphocyte ratio; BLR, band neutrophil-to-lymphocyte ratio; BLNR, band neutrophil-to-neutrophil-to-lymphocyte ratio). A review of the medical records was conducted searching...
Source: Research in Veterinary Science - August 29, 2019 Category: Veterinary Research Source Type: research

Plasma diamine oxidase activity decline with diarrhea severity in calves indicating systemic dysfunction related to intestinal mucosal damage
Publication date: Available online 27 August 2019Source: Research in Veterinary ScienceAuthor(s): Tatsuya Fukuda, Kenji Tsukano, Hiroki Nakatsuji, Kazuyuki SuzukiAbstractThe aim of the present study was to investigate whether abnormalities in plasma diamine oxidase (DAO) activity reflect the degree of intestinal mucosal disorder in calves with diarrhea. A total of 50 Holstein calves were enrolled. Thirty-six of the 50 calves presented diarrhea and were sub-classified by severity based on fecal status (0: firm, 1: pasty, 2: loose, and 3: watery) and blood pH (acidemia: blood pH
Source: Research in Veterinary Science - August 28, 2019 Category: Veterinary Research Source Type: research

Molecular characterization and computational structure prediction of activin receptor type IIB in aseel and broiler chicken
Publication date: Available online 27 August 2019Source: Research in Veterinary ScienceAuthor(s): P. Guru Vishnu, T.K. Bhattacharya, Bharat Bhushan, Pushpendra Kumar, R.N. Chatterjee, Chandan Paswan, A. Rajendra Prasad, D. Divya, K. DushyanthAbstractThe present study was formulated to characterize and comprehend the molecular structural characteristics of ACTRIIB receptor in Aseel and control broiler (CB) populations. The full length coding sequence (1539 bp) of the receptor was amplified, cloned, sequenced and analyzed using bioinformatic tools. The physico chemical properties of protein and structural features like sec...
Source: Research in Veterinary Science - August 28, 2019 Category: Veterinary Research Source Type: research

Short term impacts of meglumine antimoniate treatment on kidney function in dogs with clinical leishmaniosis
This study examines correlations among serum proteins, clinical score, body weight and kidney function biomarkers after a standard treatment course (meglumine antimoniate plus allopurinol) in twelve Canine leishmaniosis (CanL) patients at the three times points pre treatment, after treatment and after the end of treatment.The laboratory variables measured were those used for the follow-up of sick dogs along with biomarkers of kidney function: glomerular filtration rate (GFR), creatinine (Cr), urea, calcium, inorganic phosphorus, urine specific gravity (USG) and urine protein to creatinine ratio (UPC). Arterial blood pressu...
Source: Research in Veterinary Science - August 28, 2019 Category: Veterinary Research Source Type: research

Measurements of endogenous thrombin potential using the CAT method in cats: Reference values and influence of the direct factor Xa inhibitor apixaban
Publication date: Available online 28 August 2019Source: Research in Veterinary ScienceAuthor(s): Reinhard Mischke, Maike Teuber, Andreas TiedeAbstractThe aim of this study was to establish a thrombin generation assay (calibrated automated thrombogram, CAT) in cats by establishing precision (repeatability), reference values, and sensitivity to anticoagulant treatment with the factor Xa inhibitor apixaban. The CAT method was performed on citrated plasma with different commercial tissue factor (TF) reagents (PPP Reagent 1 pM [LOW], PPP Reagent 5 pM, PPP Reagent 20 pM [HIGH]) according to the manufacturers` test instruction.M...
Source: Research in Veterinary Science - August 28, 2019 Category: Veterinary Research Source Type: research

Editorial Board
Publication date: August 2019Source: Research in Veterinary Science, Volume 125Author(s): (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - August 23, 2019 Category: Veterinary Research Source Type: research

The chemical and ultra-structural analysis of thin plastic films used for surgical attenuation of portosystemic shunts in dogs and cats
The objective of the study was to (1) characterize and compare the chemical composition at the surface, subsurface and in the bulk of thin plastic films used for portosystemic shunt attenuation in their native state and after plasma exposure. (2) Assess the presence, concentration and location of irritant compounds (e.g dicetyl phosphate) within the films.Attenuated Total Reflectance Infrared Spectroscopy (ATR-IR), X-ray Photoelectron Spectroscopy (XPS) and dynamic Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) were used to analyze thirteen thin plastic films. Sample thickness was visualized and measured using S...
Source: Research in Veterinary Science - August 23, 2019 Category: Veterinary Research Source Type: research

Corrigendum to “Effect of hydrogen-rich saline on apoptosis induced by hepatic ischemia reperfusion upon laparoscopic hepatectomy in miniature pigs” [Research in Veterinary Science, Volume 119, August 2018, Pages 285–291]
Publication date: October 2019Source: Research in Veterinary Science, Volume 126Author(s): Qianzhen Zhang, Yansong Ge, Hui Li, Ge Bai, Zhihui Jiao, Xiangdong Kong, Weijing Meng, Hongbin Wang (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - August 19, 2019 Category: Veterinary Research Source Type: research

Cornelian cherry fruit improves glycaemia and manifestations of diabetes in obese Zucker diabetic fatty rats
Publication date: Available online 13 August 2019Source: Research in Veterinary ScienceAuthor(s): Marcela Capcarova, Anna Kalafova, Marianna Schwarzova, Monika Schneidgenova, Karol Svik, Marta Soltesova Prnova, Lukas Slovak, Anton Kovacik, Viktoria Lory, Stefan Zorad, Jan BrindzaAbstractCornelian cherry (Cornus mas L.) was in the past frequently used in Slovak Republic; meanwhile fell into oblivion despite the fact that it is known as antidiabetic supplement. However, there is no research investigated its effect on animal model of Diabetes mellitus (DM) 2 type as it is Zucker diabetic fatty (ZDF) rats. Thus, the aim of thi...
Source: Research in Veterinary Science - August 15, 2019 Category: Veterinary Research Source Type: research

Ferulic acid inhibits bovine endometrial epithelial cells against LPS-induced inflammation via suppressing NK-κB and MAPK pathway
Publication date: Available online 12 August 2019Source: Research in Veterinary ScienceAuthor(s): Peng Yin, Zhicong Zhang, Jiandong Li, Yaran Shi, Na Jin, Wenshu Zou, Qian Gao, Wen Wang, Fenghua LiuAbstractEndometritis is one of the most common reproductive diseases caused by bacterial infection in the cow. Ferulic acid is a major effective component extracted from Ligusticum wallichii. Ferulic acid displays pharmacological effects such as anti-inflammation and antioxidation. The aim of the present study was to investigate the protective effects of ferulic acid on inflammation induced by lipopolysaccharide (LPS) in bovine ...
Source: Research in Veterinary Science - August 13, 2019 Category: Veterinary Research Source Type: research

Antiviral activity of PHA767491 on Caprine alphaherpesvirus 1 in vitro
Publication date: Available online 12 August 2019Source: Research in Veterinary ScienceAuthor(s): Gianvito Lanave, Maria S. Lucente, Pietro Siciliano, Claudia Zizzadoro, Paolo Trerotoli, Vito Martella, Canio Buonavoglia, Maria Tempesta, Michele CameroAbstractCaprine alphaherpesvirus 1 (CpHV-1) induces genital lesions in its natural host similar to those caused by Human alphaherpesvirus 2 (HHV-2), commonly named herpes simplex virus 2 (HSV-2) in human patients. CpHV-1 infection in goats could represent a useful homologous animal model for the study of HSV-2 infection, chiefly for the assessment of antiviral drugs in in vivo...
Source: Research in Veterinary Science - August 13, 2019 Category: Veterinary Research Source Type: research

An improved system for constructing marker-free recombinant goatpox viruses to express foreign proteins
In this study, our previous GTPV-generating system was modified by reconstruction of a Loxp-containing transfer plasmid for deleting markers using the Cre/Loxp system. Meanwhile, the previous method was significantly improved by introduction of an immortalized goat testis cell line as a substitute for primary cells. Based on the latest system, a marker-free recombinant GTPV was reconstructed for expressing the EG95 antigen, and showed neither a significant difference in replication kinetics from its parental virus nor a mutation in the foreign sequence during serial 10 passages in vitro. (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - August 13, 2019 Category: Veterinary Research Source Type: research

Genes conferring resistance to critically important antimicrobials in Salmonella enterica isolated from animals and food: A systematic review of the literature, 2013–2017
Publication date: Available online 13 August 2019Source: Research in Veterinary ScienceAuthor(s): Pietro Antonelli, Simone Belluco, Marzia Mancin, Carmen Losasso, Antonia RicciAbstractAntimicrobial resistance is a major public health concern, and food systems are a crucial point in the epidemiology of these resistances. Among antimicrobials, critically important ones are therapeutic drugs that should be primarily safeguarded to allow successful outcomes against important bacterial infections in humans. The most important source of antimicrobial resistance has been recognized in the inappropriate use of antimicrobials in hu...
Source: Research in Veterinary Science - August 13, 2019 Category: Veterinary Research Source Type: research

Inclusion of Madagascar cockroach (Gromphadorhina portentosa) meal in the diet of cockatiels (Nymphicus hollandicus) in captivity: Influences on offspring development
The objective was to evaluate the inclusion of Madagascar cockroach (Gromphadorhina portentosa) meal in the diet of cockatiel (Nymphicus hollandicus) chicks in captivity. Twenty-eight cockatiel chicks were used during 90 days of experiment. The animals were divided into two groups: a control group (receiving commercial feed + seed mixture) and a test group (receiving a control diet supplemented with Madagascar cockroach meal). The cockroach meal was mixed into commercial feed at a ratio of 14:1 (commercial feed: cockroach meal; 6.6% inclusion). Parents of the chicks were fed the experimental diets 30 days before eg...
Source: Research in Veterinary Science - August 13, 2019 Category: Veterinary Research Source Type: research

Radiographic determination of humeroradial, humeroulnar, and radioulnar subluxation indices to quantify elbow incongruence in dogs confirmed to have medial coronoid disease
Publication date: Available online 13 August 2019Source: Research in Veterinary ScienceAuthor(s): Ayman A. Mostafa, Ingo Nolte, Patrick WefstaedtAbstractMedial coronoid disease and elbow incongruence are common orthopedic problems causing forelimb lameness in large breed dogs. Therefore, the objective of this retrospective clinical study is to radiographically quantify the incongruity of humeroradial, humeroulnar, and radioulnar joints in dogs with confirmed medial coronoid disease compared to controls. Sixty-six (93 elbows) control and 73 (113 elbows) diseased Labrador and Golden Retrievers were enrolled. Our objective wa...
Source: Research in Veterinary Science - August 13, 2019 Category: Veterinary Research Source Type: research

Tumor-infiltrating CD4+ and CD8+ lymphocytes and macrophages are associated with prognostic factors in triple-negative canine mammary complex type carcinoma
This study aimed to evaluate the association of CD3+, CD4+, and CD8+ T cells and tumor-infiltrating macrophages (TIMs) with the clinical parameters of female dogs harboring mammary gland tumors. Thirty female dogs affected with mammary carcinomas were used, and all tumors were histologically classified as complex carcinoma and were triple-negative phenotype determined by immunohistochemistry. Freshly frozen sections were used to determine CD3+, CD4+ and CD8+ T cells by immunohistochemistry, and TIMs were determined by immunofluorescence assays. Ten out of the 30 dogs showed lymph node metastasis at diagnosis. Fifteen dogs ...
Source: Research in Veterinary Science - August 13, 2019 Category: Veterinary Research Source Type: research

Diagnostic markers selected by immunoproteomics and phage display applied for the serodiagnosis of canine leishmaniosis
Publication date: Available online 8 August 2019Source: Research in Veterinary ScienceAuthor(s): Juliana M. Machado, Lourena E. Costa, Daniel S. Dias, Patricia A.F. Ribeiro, Vívian T. Martins, Daniela P. Lage, Gerusa B. Carvalho, Michelle L. Franklin, Grasiele S.V. Tavares, João A. Oliveira-da-Silva, Amanda S. Machado, Luana S. Ramos, Lais M. Nogueira, Reysla M.S. Mariano, Henrique B. Moura, Eduardo S. Silva, Rafael G. Teixeira-Neto, Mariana Campos-da-Paz, Alexsandro S. Galdino, Eduardo A.F. CoelhoAbstractCanine leishmaniosis (CanL) is one of the most important parasitic diseases found in several countries wo...
Source: Research in Veterinary Science - August 9, 2019 Category: Veterinary Research Source Type: research

A reply to the comment on “control of bovine mastitis in the 21st century: Immunize or tolerize?” by Fernando N. Souza and co-workers
Publication date: Available online 8 August 2019Source: Research in Veterinary ScienceAuthor(s): M. Amadori, F. Addis, V. Bronzo, B. Castiglioni, P. Moroni, R. Piccinini (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - August 9, 2019 Category: Veterinary Research Source Type: research

Letters to the editor: A comment on “Control of bovine mastitis in the 21st century: Immunize or tolerate?”
Publication date: Available online 8 August 2019Source: Research in Veterinary ScienceAuthor(s): Fernando N. Souza, Eduardo M.R. Sanchez, Alice M.M.P. Della Libera, Marcos B. Heinemann (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - August 9, 2019 Category: Veterinary Research Source Type: research

Are we ready for the big change in the dairy production system?
Publication date: Available online 7 August 2019Source: Research in Veterinary ScienceAuthor(s): Alice Brombin, Andrea Pezzuolo, Marta Brščić (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - August 9, 2019 Category: Veterinary Research Source Type: research

Recent progress in biomedical applications of chitosan and its nanocomposites in aquaculture: A review
Publication date: Available online 7 August 2019Source: Research in Veterinary ScienceAuthor(s): Fatma Ahmed, Faiza M. Soliman, Mohamed A. Adly, Hamdy A.M. Soliman, Mansour El-Matbouli, Mona SalehAbstractChitosan nanoparticles (CSNPs) are the nanostructures of chitosan biopolymer which is derived from chitin polysaccharide, the main component of crustacean shells. Chitosan is a biocompatible, nontoxic and biodegradable polymer soluble in acidic solutions and easily excreted from kidneys. It is widely used in medical and pharmaceutical applications including artificial matrices for tissue engineering, drug transport, target...
Source: Research in Veterinary Science - August 9, 2019 Category: Veterinary Research Source Type: research

Assessment of potential neuropathic changes in cattle after cautery disbudding
Publication date: Available online 6 August 2019Source: Research in Veterinary ScienceAuthor(s): B. Vidondo, S. Stettler, A. Stojiljkovic, H. Mogel, V. Gaschen, C. Spadavecchia, D. Casoni, M.H. StoffelAbstractDisbudding of calves is a standard husbandry procedure to reduce the risk of injuries to other cattle and to workers. Whereas acute pain resulting from disbudding has been studied extensively, little is known about chronic pain as a potential long-term consequence. The goal of the present study was to investigate possible morphological changes in the cornual nerve as a function of disbudding. Samples were collected fr...
Source: Research in Veterinary Science - August 7, 2019 Category: Veterinary Research Source Type: research

Correlation of rotational thromboelastometry (ROTEM) parameters with platelet count and their ability to predict thrombocytopenia in dogs
Publication date: Available online 6 August 2019Source: Research in Veterinary ScienceAuthor(s): Jennifer S. Turner, Annette P.N. Kutter, Nadja E. SigristAbstractThe study objectivs were to evaluate the correlation between platelet count (PLT) and rotational thromboelastometry (ROTEM) parameters and to determine ROTEM cut-off values for identification of thrombocytopenia in dogs. Medical records of 113 dogs with concurrent EXTEM (ROTEM activated by proprietary tissue factor), FIBTEM (EXTEM with added cytochalasin D) analysis and PLT were retrospectively reviewed. Signalment, treatment prior to analysis, hematocrit (HCT), E...
Source: Research in Veterinary Science - August 7, 2019 Category: Veterinary Research Source Type: research

Amelioration effects of Kaempferol on immune response following chronic intermittent cold-stress
This study investigated the effects of KPF on immune factors and intestinal antioxidation in the blood of cold-stressed mice.KPF was solubilized in diluted saline before administration. The mice were randomly divided into 4 groups: (1) control, (2) cold-stress, (3) KPF 25 mg/kg, and (4) cinnamon (CAM) 30 mg/kg groups. Groups (2)–(4) were exposed to cold stress once a day for 7 days. Cold-stress was achieved by exposing the mice to a temperature of −15 °C and 70 ± 10% humidity for 60 min, once a day. The histopathological changes in the small intestine of the mice were analyzed. The T ly...
Source: Research in Veterinary Science - August 7, 2019 Category: Veterinary Research Source Type: research

Application of a score for evaluation of pain, distress and discomfort in pigs with lameness and prolapses: Correlation with saliva biomarkers and severity of the disease
Publication date: Available online 6 August 2019Source: Research in Veterinary ScienceAuthor(s): María Dolores Contreras-Aguilar, Damián Escribano, Silvia Martínez-Miró, Marina López-Arjona, Camila P. Rubio, Silvia Martínez-Subiela, José J. Cerón, Fernando TeclesAbstractA score system was used to evaluate pain, distress and discomfort in healthy pigs and pigs with two different diseases: lameness and rectal prolapse. In order to evaluate if the score was repetitive, 10 pigs were scored by three different researchers twice with a gap of 3 hours between, achieving repet...
Source: Research in Veterinary Science - August 7, 2019 Category: Veterinary Research Source Type: research

The impact of heat stress on the immune system in dairy cattle: A review
Publication date: Available online 6 August 2019Source: Research in Veterinary ScienceAuthor(s): M. Bagath, G. Krishnan, C. Devaraj, V.P. Rashamol, P. Pragna, A.M. Lees, V. SejianAbstractHeat stress is well documented to have a negative influence on livestock productivity and these impacts may be exacerbated by climate change. Dairy cattle can be more vulnerable to the negative effects of heat stress as these adverse impacts may be more profound during pregnancy and lactation. New emerging diseases are usually linked to a positive relationship with climate change and the survival of microrganisms and/or their vectors. Thes...
Source: Research in Veterinary Science - August 7, 2019 Category: Veterinary Research Source Type: research

Cardiopulmonary nematode infections in wild canids: Does the key lie on host-prey-parasite evolution?
This study revealed an association between infection and environmental factors, and highlighted a wide variation in the spatial distribution of A. vasorum. Our results indicate that cardiopulmonary parasites are widespread in wild canids in northwest Spain, and further agrees with other studies indicating the expansion of A. vasorum in Europe and, therefore, the urgent need to investigate infection in dogs in sympatric areas. (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - August 7, 2019 Category: Veterinary Research Source Type: research

Histological and morphometric study of the components of the sinus and atrioventricular nodes in horses and dogs
Publication date: Available online 5 August 2019Source: Research in Veterinary ScienceAuthor(s): F.A. Gómez-Torres, L.E. Ballesteros-Acuña, A. Ruíz-SauriAbstractThe cardiac nodes are the source of the electrical impulse that is transmitted to the heart, the aim of this work is study the histological and morphometric characteristics of the different components of the sinus and atrioventricular nodes in horses and dogs that help to know the physiopathology of these nodes.A group of ten horse hearts and five dog hearts were used. The region of the sinus and atrioventricular nodes was sectioned serially, a...
Source: Research in Veterinary Science - August 6, 2019 Category: Veterinary Research Source Type: research

Establishment of BV2 microglia polarization model and its effect on Toxoplasma gondii proliferation
Publication date: Available online 5 August 2019Source: Research in Veterinary ScienceAuthor(s): Longtao Wang, Qiang Liu, Yongli Zhang, Lisi Chen, Shouping Hu, Zhuo Zhang, Jingfei Wang, Xuehui Cai, Honglin Jia, Xijun HeAbstractToxoplasma gondii is an intracellular opportunistic, parasitic protozoan. Microglia have been classified into two main types: M1 (classically activated macrophages) and M2 (alternatively activated macrophages). BV2 cells were used in this study, together with lipopolysaccharide (LPS) and interferon (IFN)-γ or interleukin (IL)-4, which were used to induce resting microglia. Expression levels of ...
Source: Research in Veterinary Science - August 6, 2019 Category: Veterinary Research Source Type: research

Echocardiographic assessment of left ventricular systolic function in neonatal calves with naturally occurring sepsis or septic shock due to diarrhea
The objectives of this study were to evaluate the clinical value of echocardiography for monitoring left ventricular (LV) systolic function in septic calves. A prospective longitudinal study was performed using a convenience sample. Twenty septic calves and 10 healthy calves were enrolled in the study. Arterial blood pressure (BP) was measured and M-mode echocardiography performed to characterize LV systolic function; the latter included measurement of ejection fraction, EF; stroke volume, SVI and cardiac output indexed to body weight, CI; E-point of septal separation, EPSS; pre-ejection period, PEP; ejection time, LVET; r...
Source: Research in Veterinary Science - August 6, 2019 Category: Veterinary Research Source Type: research

A new highly sensitive immunoassay for the detection of adiponectin in serum and saliva of dogs and its application in obesity and canine leishmaniosis
Publication date: Available online 1 August 2019Source: Research in Veterinary ScienceAuthor(s): A. Muñoz-Prieto, S. Martínez-Subiela, J. Cerón, A. TvarijonaviciuteAbstractAdiponectin is an adipokine that exerts insulin-sensitizing and antiinflammatory properties. The aim of this study was to develop and validate a new heterologous ultrasensitive assay based on amplified luminescent technology for adiponectin determination in serum and saliva of dogs. A complete analytical validation of the assay was made in these fluids, and also this assay was applied to quantify adiponectin in serum and saliva of ob...
Source: Research in Veterinary Science - August 2, 2019 Category: Veterinary Research Source Type: research

Prospective randomized comparison of platelet rich plasma to extracorporeal shockwave therapy for treatment of proximal suspensory pain in western performance horses
Publication date: Available online 1 August 2019Source: Research in Veterinary ScienceAuthor(s): Kelly Giunta, Josh R. Donnell, Alan D. Donnell, David D. FrisbieAbstractProximal suspensory desmitis (PSD) is a frequent cause of lameness in the performance horse. Extracorporeal shockwave therapy (EST) and platelet rich plasma (PRP) have become common treatments for PSD yet clinical data on efficacy is limited. The main objective of this randomized, prospective clinical study was to compare long-term effectiveness of EST and PRP in Western performance horses. One hundred horses with lameness localized to the proximal suspenso...
Source: Research in Veterinary Science - August 2, 2019 Category: Veterinary Research Source Type: research

Adding α-tocopherol-selenium and ascorbic acid to periparturient sow diets influences hemogram, lipid profile, leptin, oxidant/antioxidant imbalance, performance and neonatal piglet mortality
Publication date: Available online 23 July 2019Source: Research in Veterinary ScienceAuthor(s): Chandra Kiran Gaykwad, Ujjwal Kumar De, Sunil E. Jadhav, G.E. Chethan, Akhilesh, Nihar Ranjan Sahoo, D.B. Mondal, G.K. Gaur, Med Ram Verma, Pallab ChaudhuriAbstractAlpha-tocopherol-selenium (ATS) and ascorbic acid (AA) are the potent antioxidants. The present study investigated whether supplementation of ATS and AA in periparturient sows has positive effects on amelioration of oxidative stress, serum immunoglobulin G (IgG), lipid profile and sows performance. For this, twenty-four pregnant multiparous sows (landrace×indige...
Source: Research in Veterinary Science - July 25, 2019 Category: Veterinary Research Source Type: research

Minthostachys verticillata essential oil activates macrophage phagocytosis and modulates the innate immune response in a murine model of Enterococcus faecium mastitis
Publication date: Available online 19 July 2019Source: Research in Veterinary ScienceAuthor(s): Ivana Dalila Montironi, Elina BeatrizReinoso, Valentina Croce Paullier, María Inés Siri, María Julia Pianzzola, Melina Moliva, Noelia Campra, Guillermo Bagnis, Isabel Ferreira LaRocque-de-Freitas, Debora Decote-Ricardo, Celio Geraldo Freire-de-Lima, José María Raviolo, Laura Noelia CariddiAbstractMinthostachys verticillata essential oil (EO) is a natural product that reports immunomodulatory effects on human T cells as well as anti-inflammatory activity. Bovine mastitis is a worldwide disease, ...
Source: Research in Veterinary Science - July 20, 2019 Category: Veterinary Research Source Type: research

Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus
Publication date: Available online 19 July 2019Source: Research in Veterinary ScienceAuthor(s): Svetlana Nedić, SlobodankaVakanjac, Marko Samardžija, Sunčica BorozanAbstractThe aim of this study was to determine serum lipid values and parameters of oxidative stress in blood and milk of cows with subclinical mastitis (SCM) caused by Staphylococcus aureus and to establish association between these parameters. The study was performed on total of 104 cows assigned into control group of healthy animals (n = 12) and two groups of cows with SCM, either SCM1 group (n = 37) with ˂ 1000 CFU/mL of S. aureus or SCM2 grou...
Source: Research in Veterinary Science - July 20, 2019 Category: Veterinary Research Source Type: research

Clinical efficacy of clodronic acid in horses diagnosed with navicular syndrome: A field study using objective and subjective lameness evaluation
This study demonstrates that intramuscular clodronic acid can be useful for lameness reduction in some horses with navicular syndrome. (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - July 19, 2019 Category: Veterinary Research Source Type: research

Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows
This study evaluated and compared infrared thermography (IRT) and rectal temperature (RT) as screening tests to identify sick transition dairy cows. Holstein cows (n = 72; 42 primiparous) had RT and IRT temperatures taken daily from 1 to 12 days in milk (DIM). Health examinations were performed daily to diagnose retained fetal membrane, milk fever and metritis, and blood was analyzed for β-hydroxybutyrate at 6 and 9 DIM to diagnose ketosis. Plasma concentrations of cortisol, interleukin-6, tumor necrosis factor α and serum amyloid A at 3, 6, 9 and 12 DIM were included as additional indicators of illness. C...
Source: Research in Veterinary Science - July 19, 2019 Category: Veterinary Research Source Type: research

Effect of feeding on the pharmacokinetics of vilazodone in dogs
In conclusion, feeding can affect the pharmacokinetics of VLZ in the dog. (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - July 18, 2019 Category: Veterinary Research Source Type: research

Bleeding diathesis in canine multiple myeloma and prognostic implications: A cohort study in 156 dogs
Publication date: Available online 12 July 2019Source: Research in Veterinary ScienceAuthor(s): Marco Caldin, Michela Campigli, Andrea Zoia, Anna Zanella, Giovanna Bertolini, Tommaso Furlanello, George LubasAbstractMultiple myeloma (MM) is a tumor of plasma cells representing approximately 1% of all canine tumors. Clinical evident bleeding is often referred to as the main finding. The aim of the study was to evaluate the occurrence of clinical bleedings in dogs with MM and its prognostic implications compared to a population of dogs not affected by MM.Two groups of dogs (# 78 each) individually matched for breed, age and g...
Source: Research in Veterinary Science - July 14, 2019 Category: Veterinary Research Source Type: research

Serum Apelin-36 alteration in late pregnancy and early lactation of dairy cows and its association with negative energy balance markers
In conclusion, our results showed that serum Apelin-36 concentration decreased after parturition in dairy cow. Alteration of Apelin-36 secretion after parturition may represent an endocrine adaptation in dairy cow during the lactating period. (Source: Research in Veterinary Science)
Source: Research in Veterinary Science - July 14, 2019 Category: Veterinary Research Source Type: research