Evaluation of commonly used tests to measure the effect of single-dose aspirin on mouse hemostasis

Acetyl salicylic acid (ASA or aspirin) at low dose is broadly used for its antiplatelet effect in primary and secondary prevention of cardiovascular diseases [1]. ASA irreversibly acetylates the Ser529 residue of COX1, thereby leading to a steric hindrance of the COX channel that prevents access of the substrate to the catalytic site of the enzyme [2]. COX1 is responsible for the conversion of arachidonic acid into prostaglandins, that are precursors of thromboxane A2 (TXA2) in platelets [3]. By binding the thromboxane and prostanoid receptor (TP receptor), platelet-secreted TXA2 enhances platelet activation initially triggered by agonists such as collagen.

Source: Prostaglandins, Leukotrienes and Essential Fatty Acids - August 7, 2019 Category: Biomedical Science Authors: Benoit Decouture, Alexandre Leuci, Blandine Dizier, Tiphaine Belleville-Rolland, Alexandre Mansour, Fanny Martin, Dominique Pidard, Pascale Gaussem, Christilla Bachelot-Loza Source Type: research