Effects of Resveratrol Postconditioning on Cerebral Ischemia in mice: Role of the Sirtuin-1 (SIRT1) Pathway.

Effects of Resveratrol Postconditioning on Cerebral Ischemia in mice: Role of the Sirtuin-1 (SIRT1) Pathway.
Can J Physiol Pharmacol. 2019 Jul 24;:
Authors: Grewal AK, Singh N, Singh TGT
Abstract
Evidence has demonstrated that resveratrol preconditioning exhibits neuroprotection against cerebral IR injury. The current investigation aimed to explore whether pharmacological postconditioning, by administering resveratrol, after a sustained ischemia & prior to prolonged reperfusion abrogates cerebral IR injury. Cerebral ischemia-reperfusion-induced injury mice model was employed in this study to evaluate the neuroprotective effects of pharmacological postconditioning (pPoCo) with resveratrol (30 mg/kg; i.p.) administered 5 mins before reperfusion. We administered Sirtinol, a SIRT1/2 selective inhibitor (10 mg/kg; i.p.) 10 min before ischemia (17 min) and reperfusion (24 h), to elucidate whether the neuroprotection with resveratrol postconditioning depends on SIRT1 activation. Various biochemical, behavioral parameters and histopathological changes were assessed to examine the effect of pPoCo. Infarct size is estimated using TTC staining. It was established that resveratrol postconditioning abrogated the deleterious effects of IR injury expressed with regard to biochemical parameters of oxidative stress (TBARS, SOD, GSH), acetylcholinestrase activity, behavioual parameters (memory, motor coordination), infarct size and histopathologic...

Source: Canadian Journal of Physiology and Pharmacology - July 23, 2019 Category: Drugs & Pharmacology Authors: Grewal AK, Singh N, Singh TGT Tags: Can J Physiol Pharmacol Source Type: research