Paxillin Regulated Genomic Networks in Prostate Cancer

Publication date: Available online 22 July 2019Source: SteroidsAuthor(s): Xiaoting Ma, Anindita Biswas, Stephen R. HammesAbstractPaxillin is extensively involved in focal adhesion signaling and kinase signaling throughout the plasma membrane and cytoplasm. However, recent studies in prostate cancer suggest that paxillin also plays a critical role in regulating gene expression within the nucleus, serving as a liaison between cytoplasmic and nuclear MAPK and Androgen Receptor (AR) signaling. Here we used RNA-seq to examine the transcriptome in several human prostate cancer cell lines. First, we examined paxillin effects on androgen-mediated transcription in control or paxillin-depleted AR-positive LNCaP and C4-2 human prostate cancer cells. In androgen-dependent LNCaP cells, we found over 1000 paxillin-dependent androgen-responsive genes, some of which are involved in endocrine therapy resistance. Most paxillin-dependent AR-mediated genes in LNCaP cells were no longer paxillin-dependent in androgen-sensitive, castration-resistant C4-2 cells, suggesting that castration-resistance may markedly alter paxillin effects on genomic AR signaling. To examine the paxillin-regulated transcriptome in the absence of androgen signaling, we performed RNA-seq in AR-negative PC3 human prostate cancer cells. Paxillin enhanced several pro-proliferative pathways, including the CyclinD/Rb/E2F and DNA replication/repair pathways. Additionally, paxillin suppressed pro-apoptotic genes, including CASP1...
Source: Steroids - Category: Drugs & Pharmacology Source Type: research

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Authors: Qiu Y, Yang S, Pan T, Yu L, Liu J, Zhu Y, Wang H Abstract Prostate cancer is a common malignant tumor in elderly men. As a novel metabolic-reprogramming molecule, the role of ankyrin repeat domain 22 (ANKRD22) in the tumorigenesis and progression of prostate cancer remains unknown. In the present study, mouse monoclonal antibodies against human ANKRD22 were prepared using recombinant ANKRD22 from prokaryotic expression and validated. Subsequently, these antibodies were used to evaluate ANKRD22 levels via immunohistochemical staining in prostate cancer tissues. Finally, the association between ANKRD22 level...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
lncRNA MIR4435-2HG promotes cancer cell migration and invasion in prostate carcinoma by upregulating TGF-β1. Oncol Lett. 2019 Oct;18(4):4016-4021 Authors: Zhang H, Meng H, Huang X, Tong W, Liang X, Li J, Zhang C, Chen M Abstract The long noncoding (lnc) RNA MIR4435-2HG is known to promote lung cancer; however, its role in prostate carcinoma (PCa) remains unknown. The aim of the current study was therefore to investigate the role of MIR4435-2HG in PCa by detecting differential gene expression using quantitative PCR and ELISA kits. Furthermore, overexpression experiments were performed to analyze ge...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
Authors: Shi Z, Guo F, Jia D, Huang J, Chen J, Sun M, Qi F, Liang C Abstract A previous study reported the decreased expression of long non-coding RNA mortal obligate RNA transcript (lncRNA MORT) in 16 types of cancer, while the functionality of lncRNA MORT in cancer biology remains unknown. Therefore, the present study was conducted to characterize the functionality of lncRNA MORT in prostate carcinoma, a common cancer type worldwide. lncRNA MORT expression level was downregulated in tumor tissues compared with that in the adjacent healthy tissues of patients with prostate carcinoma. Expression of lncRNA MORT in t...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
STEPHEN FRY is one of the nation ’s fondest figures. The multi-talented actor shocked his fanbase last year when he took to Twitter to reveal he had been diagnosed with prostate cancer. The actor, who has now made a full recovery, recounted the events in minute detail to raise awareness.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
Two prostate cancer risk calculators that estimate the probability of reclassification with or without biopsy were validated in five large cohorts, researchers say - but using the calculators in the clinic can be complicated.Reuters Health Information
Source: Medscape Pathology Headlines - Category: Pathology Tags: Hematology-Oncology News Source Type: news
Publication date: Available online 12 September 2019Source: SteroidsAuthor(s): Debbie O'Reilly, Patricia Johnson, Paul.J BuchananAbstractAndrogen deprivation therapy (ADT) is the main treatment to prolong survival in advance stage prostate cancer (PCa) but associated resistance leads to the development of terminal castrate resistant PCa (CRPC). Current research demonstrates a critical role of prostate cancer stem cells (PCSC) in the development of treatment resistance and subsequent disease progression. Despite uncertainty surrounding the origin of these cells, studies clearly show they are associated with poorer outcomes ...
Source: Steroids - Category: Drugs & Pharmacology Source Type: research
Every year, tens thousands of men in the United States are diagnosed with low-risk prostate cancer. Most are told that they don ’t need treatment other than “active surveillance” to monitor their slow-growing tumors. Now, UCLA physicians have shown that the best way to proceed with this is by starting out with an MRI-guided prostate biopsy.In the past, doctors relied on ultrasound to guide a biopsy needle into the prostate. However, ultrasound doesn ’t allow the visualization of prostate tumors, so a biopsy may have missed a tumor. Over the past decade, it’s become possible to instead perform ...
Source: UCLA Newsroom: Health Sciences - Category: Universities & Medical Training Source Type: news
Ulrika Rosenström Simultaneous targeting of the prostate-specific membrane antigen (PSMA) and gastrin-releasing peptide receptor (GRPR) could improve the diagnostic accuracy in prostate cancer (PCa). The aim of this study was to develop a PSMA/GRPR-targeting bispecific heterodimer for SPECT and positron emission tomography (PET) diagnostic imaging of PCa. The heterodimer NOTA-DUPA-RM26 was produced by manual solid-phase peptide synthesis. NOTA-DUPA-RM26 was labeled with 111In and 68Ga, with yields >98%, and demonstrated a high stability and binding specificity to PSMA and GRPR. IC50 values for natIn-NOTA-DU...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
Alejandro S. Godoy Tissue homeostasis is the result of a complex intercellular network controlling the behavior of every cell for the survival of the whole organism. In mammalian tissues, cells do communicate via diverse long- and short-range communication mechanisms. While long-range communication involves hormones through blood circulation and neural transmission, short-range communication mechanisms include either paracrine diffusible factors or direct interactions (e.g., gap junctions, intercellular bridges and tunneling nanotubes) or a mixture of both (e.g., exosomes). Tumor growth represents an alteration of tis...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Review Source Type: research
ConclusionOur results confirm the validity of the ERSPC RC in a South African cohort. Application of this calculator to the wider South African population would allow better selection of patients for prostate biopsy and spare a significant number its adverse consequences.
Source: World Journal of Urology - Category: Urology & Nephrology Source Type: research
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