25-Hydroxyvitamin D3 positively regulates periodontal inflammaging via SOCS3/STAT signaling in diabetic mice
ConclusionDiabetic periodontitis appeared to develop an inflammaging status resulted in periodontal infection. 25(OH)D3 could inhibit SASP secretion through reducing SOCS3 expression in experimental diabetic periodontitis, dependently inactivating NF-κB pro-inflammatory signaling. The reversible effect further documented that the inflammaging might be a highly likely contributor in diabetic periodontitis.Graphical abstract25(OH)D3 played the protective role in gingival tissue of db/db mice with periodontitis. SOCS3 is an endogenous negative regulator of the inflammaging. Diabetic periodontitis developed an inflammagi...
Source: Steroids - January 7, 2020 Category: Drugs & Pharmacology Source Type: research

Training counteracts DEX-induced microvascular rarefaction by improving the balance between apoptotic and angiogenic proteins
Publication date: Available online 2 January 2020Source: SteroidsAuthor(s): Isley de Jesus, Naiara A. Herrera, Jesus C. Andreo, Carlos F. Santos, Sandra L. AmaralAbstractThis work investigated the mechanisms induced by exercise training that may contribute to attenuate dexamethasone (DEX)-induced microvascular rarefaction and hypertension. Wistar rats underwent training protocol or were kept sedentary for 8 weeks. Dexamethasone was administered during the following 14-days and hemodynamic parameters were recorded at the end. Capillary density (CD) and capillary-to-fiber ratio (C:F ratio) were obtained in soleus muscle (SOL...
Source: Steroids - January 4, 2020 Category: Drugs & Pharmacology Source Type: research

Is 9β-dehydrohalogenation of betamethasone and dexamethasone hindering the detection of banned co-eluting meprednisone? A reverse-phase chiral liquid chromatography high-resolution mass spectrometry approach
Publication date: Available online 3 January 2020Source: SteroidsAuthor(s): Tajudheen K. Karatt, M. Anwar Sathiq, Saraswathy LayaAbstractMass spectral analyses of dexamethasone and betamethasone reveal intense signals at m/z 373.19994 (using a Thermo Q Exactive high-resolution mass spectrometer coupled with Dionex UltiMate 3000 UHPLC+ operated in the positive ion mode), matching the signal of meprednisone, the 11-oxo version of methylprednisolone, along with its parent signal; possibly due to dehydrohalogenation of these drugs at MS. The parent mass of meprednisone is exactly same as that of dehydrohalogenated mass of dexa...
Source: Steroids - January 4, 2020 Category: Drugs & Pharmacology Source Type: research

Evolutions in cardiac and gonadal ultra-structure during a “Cycle” of androgenic anabolic abuse in adult male mice
ConclusionThese results ported that the use of AAS with “Cycling” may lead to irreversibly destroy the heart tissue. Either, “Cycling” does not ensure the complete recovery of fertility in AAS abusers. (Source: Steroids)
Source: Steroids - January 1, 2020 Category: Drugs & Pharmacology Source Type: research

Abuse of androgenic anabolic drugs with “Cycling” induces hepatic steatosis in adult male mice
ConclusionThese results ported that the use of AAS with “Cycling” may lead to the development of hepatic steatosis before progressing to more serious pathological liver situations in AAS abusers. (Source: Steroids)
Source: Steroids - January 1, 2020 Category: Drugs & Pharmacology Source Type: research

Sexual dimorphism in the microbiology of the CF ‘Gender Gap’: Estrogen modulation of Pseudomonas aeruginosa virulence
In conclusion, we have demonstrated for the first time that estrogen exacerbates P. aeruginosa virulence factors and enhances bacterial interactions with CF bronchial epithelium which can be inhibited by tamoxifen. Our work suggests that SERMs could be used as an adjuvant treatment to block estrogen-induced P. aeruginosa infections and associated lung exacerbations in females with CF.Graphical abstract (Source: Steroids)
Source: Steroids - January 1, 2020 Category: Drugs & Pharmacology Source Type: research

Spirostanol and sesquiterpenoid glycosides from the rhizomes of Trillium tschonoskii
Publication date: Available online 30 December 2019Source: SteroidsAuthor(s): Dan Li, Huan Yan, Jie Wang, Qian Yu, Wei Ni, Lin-Kun An, Xu-Jie Qin, Hai-Yang LiuAbstractThree new spirostanol glycosides, trilliumosides K–M (1–3), one new sesquiterpenoid glycoside, tritschsesuquiside A (4), along with three known analogues (5–7) were obtained from the rhizomes of Trillium tschonoskii. The structures of new glycosides were elucidated by spectroscopic analyses (HRMS and NMR) and chemical methods. Glycosides 5–7 displayed cytotoxicities against five human cancer cell lines with IC50 values ranging from 10....
Source: Steroids - December 31, 2019 Category: Drugs & Pharmacology Source Type: research

Molecular structure study of a heptakis(2,3,6-tri-O–methyl)–β–cyclodextrin complex of cholesterol
Publication date: Available online 19 December 2019Source: SteroidsAuthor(s): Karolína Kučáková, Bohumil DolenskýAbstractHeptakis(2,3,6–tri–O–methyl)–β-cyclodextrin (2) and cholesterol form a water-soluble complex 3. We performed several NMR studies, particularly 1H, 13C, 2D NOESY and DOSY, at various temperatures on 500 and 950 MHz instruments. We discovered that the complex 3 is unstable above 57 °C in heavy water, while it is kinetically stable enough to be studied by NMR in detail at 1 °C. We demonstrated the formation of a face–to–...
Source: Steroids - December 21, 2019 Category: Drugs & Pharmacology Source Type: research

The effect of finasteride and dutasteride on the synthesis of neurosteroids by glioblastoma cells
Publication date: Available online 20 December 2019Source: SteroidsAuthor(s): Pinacho-Garcia Luis Manuel, Valdez Ricardo A, Navarrete Araceli, Cabeza Marisa, Segovia Jose, Romano Marta CAbstractGlioblastoma (GBM) is the most aggressive local brain tumor and effective treatments are lacking. Many studies have proposed an important participation of steroid hormones in the development of gliomas. Evidence was provided by statistics analysis where the incidence in adult population is 50% higher in men than in women. Female patients have a better prognosis for survival compared to male patients with GBM. Also, the expression of...
Source: Steroids - December 21, 2019 Category: Drugs & Pharmacology Source Type: research

Cytotoxic steroidal saponins from the rhizome of Anemarrhena asphodeloides
Publication date: Available online 20 December 2019Source: SteroidsAuthor(s): Yun-Fang Zhao, Jing Zhou, Min-Jie Zhang, Min Zhang, Xue-Feng HuangAbstractTwo novel steroidal saponins, timosaponin V and W (1 and 2), together with seven known steroidal saponins (3-9), were isolated from the rhizomes of Anemarrhena asphodeloides Bunge. Their structures were elucidated by extensive 1D NMR and 2D NMR (HSQC, HMBC, 1H-1H COSY, and NOESY), and MS analyses. The cytotoxic activities of the isolates were evaluated. Compound 1 showed a significant cytotoxic activity against MCF-7 and HepG2 cell lines with IC50 values of 2.16±0.19...
Source: Steroids - December 21, 2019 Category: Drugs & Pharmacology Source Type: research

Two new hydroperoxy steroids from the South China Sea gorgonian Rumphella sp.
Publication date: Available online 20 December 2019Source: SteroidsAuthor(s): Fang-Zhou Yin, Min Yang, Song-Wei Li, Meng-Jun Wu, Xia-Juan Huan, Ze-Hong Miu, Hong Wang, Yue-Wei GuoAbstractTwo new hydroperoxy steroids, namely, xidaosteroids A and B (1 and 2), along with five known related compounds 3–7, were isolated from the South China Sea gorgonian Rumphella sp.. Their structures were established by extensive spectroscopic analyses and comparison of the spectral data with those reported in the literature. In bioassay, compound 3 showed weak cytotoxicities against SNU-398 and Capan-1 cells.Graphical abstract: (Source: Steroids)
Source: Steroids - December 21, 2019 Category: Drugs & Pharmacology Source Type: research

Guest editorial: 11th international meeting on rapid responses to steroid hormones RRSH2018
Publication date: February 2020Source: Steroids, Volume 154Author(s): Brian J. Harvey (Source: Steroids)
Source: Steroids - December 18, 2019 Category: Drugs & Pharmacology Source Type: research

Steroid hormone receptors: a south american perspective
Publication date: Available online 10 December 2019Source: SteroidsAuthor(s): Patricia V. Elizalde, Cecilia J. Proietti (Source: Steroids)
Source: Steroids - December 12, 2019 Category: Drugs & Pharmacology Source Type: research

Protein kinase D2 Regulates Epithelial Sodium Channel Activity and Aldosterone Non-Genomic Responses in Renal Cortical Collecting Duct Cells
Publication date: Available online 11 December 2019Source: SteroidsAuthor(s): Warren Thomas, Ruth Dooley, Sinead Quinn, Manuel Yusef Robles, Brian J. HarveyAbstractProtein kinase D2 (PKD2) is a serine/ threonine protein kinase which plays an important role in vesicle fission at the trans-Golgi network (TGN) to coordinate subcellular trafficking with gene expression. We found that in the rat kidney, PKD2 is specifically expressed in collecting duct principal cells predominantly at the apical membrane and with lower basal expression in cytosolic compartments. When rats were maintained on a Na+ depleted diet (
Source: Steroids - December 12, 2019 Category: Drugs & Pharmacology Source Type: research

11th International Meeting on Rapid Responses to Steroid Hormones RRSH2018
Publication date: Available online 7 December 2019Source: SteroidsAuthor(s): Brian Harvey (Source: Steroids)
Source: Steroids - December 7, 2019 Category: Drugs & Pharmacology Source Type: research

Stanazolol derived ELISA as a sensitive forensic tool for the detection of multiple 17α-methylated anabolics
Publication date: Available online 5 December 2019Source: SteroidsAuthor(s): Lukáš Huml, Dominika Havlová, Ondřej Longin, Eliška Staňková, Barbora Holubová, Martin Kuchař, Elena Prokudina, Zdeňka Rottnerová, Tomáš Zimmermann, Pavel Drašar, Oldřich Lapčík, Michal JurášekAbstractTwo valuable forensic tools based on enzyme-linked immunoassays (ELISAs) for the analysis of 17α-methylated steroids were developed using haptens of stanazolol and its conjugates with biotin. Haptens containing terminal carboxylic group were conjuga...
Source: Steroids - December 6, 2019 Category: Drugs & Pharmacology Source Type: research

The role of estrogen in intervertebral disc degeneration
Publication date: Available online 6 December 2019Source: SteroidsAuthor(s): Lin-Yu Jin, Xiao-Xing Song, Xin-Feng LiAbstractIntervertebral disc degeneration (IVDD) is a main contributor to low back and radicular pain, which imposes heavy economic burdens on society. However, the etiology and mechanism of IVDD are complex and still not completely clear. In particular, the role of estrogen in IVDD has not received much attention in recent research, although estrogen plays a crucial role in the metabolic dysfunction of others musculoskeletal structures, such as bone, muscle, and tendon. In this review, we attempt to describe ...
Source: Steroids - December 6, 2019 Category: Drugs & Pharmacology Source Type: research

21-benzylidene digoxin decreases proliferation by inhibiting the egfr/erk signaling pathway and induces apoptosis in hela cells
Publication date: Available online 6 December 2019Source: SteroidsAuthor(s): Marco Túlio C. Pessôa, Jéssica M.M. Valadares, Sayonarah C. Rocha, Simone C. Silva, Jeff P. McDermott, Gladis Sánchez, Fernando P. Varotti, Cristóforo Scavone, Rosy I.M.A. Ribeiro, José A.F.P. Villar, Gustavo Blanco, Leandro A. BarbosaAbstractCardiotonic steroids (CTS) are agents traditionally known for their capacity to bind to the Na,K-ATPase (NKA), affecting the ion transport and the contraction of the heart. Natural CTS have been shown to also have effects on cell signaling pathways. With the goal of de...
Source: Steroids - December 6, 2019 Category: Drugs & Pharmacology Source Type: research

Endogenous steroid hormones in hair: investigations on different hair types, pigmentation effects and correlation to nails
In this study, a liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous identification and quantification of seven steroid hormones (cortisone, cortisol, 11-deoxycortisol, androstenedione, 11-deoxycorticosterone, testosterone, progesterone) in hair. Cortisol, cortisone, androstenedione, testosterone and progesterone were detected and quantified in authentic hair samples of different individuals. Significantly higher concentrations for body hair were found for cortisone and testosterone compared to scalp hair. Furthermore, missing correlations for the majority of steroids betw...
Source: Steroids - December 5, 2019 Category: Drugs & Pharmacology Source Type: research

Heterocyclic Steroids: Efficient Routes for Annulation of Pentacyclic Steroidal Pyrimidines
Publication date: Available online 2 December 2019Source: SteroidsAuthor(s): M. Monier, Ahmed El-Mekabaty, Doaa Abdel-Latif, Başak Doğru Mert, Khaled M. ElattarAbstractSteroids are components of cell membranes, signaling molecules and are a type of secondary metabolites as a result of their high impact of biological significance. The present review described the literature reports of pentacyclic steroidal pyrimidines as a type of heterocyclic steroids. The main sections included the synthesis of the investigated steroids fused at rings-A or B or D of steroid skeleton, synthesis of binary or linked-type pyrimidines, pyrim...
Source: Steroids - December 3, 2019 Category: Drugs & Pharmacology Source Type: research

Polyhydroxylated sterols from Monascus purpureus-fermented rice
Publication date: February 2020Source: Steroids, Volume 154Author(s): Jin-Jie Li, Xiang-Jian Zhong, Xin Wang, Xi-Miao Yang, Jian-Yu Yue, Xuan Zhang, Jia-Chen Liu, Kai-Qi Wang, Xiao-Ya ShangAbstractUsing a cell-based cytotoxicity assay, two new polyhydroxylated sterols, 16(S),22(S)-epoxy-3β,5α,6β,20(R),23(R),25-hexahydroxy-7-ergostene and 3β,7β,8α,25-tetrahydroxy-5,22E-ergostadiene were isolated from the ethyl acetate portion of the ethanolic extract of Monascus purpureus. Their structures were elucidated by spectroscopic analysis and in comparison with those reported in the literature. Both ...
Source: Steroids - November 29, 2019 Category: Drugs & Pharmacology Source Type: research

LncRNA-MALAT1 promotes osteogenic differentiation through regulating ATF4 by sponging miR-214: Implication of steroid-induced avascular necrosis of the femoral head
ConclusionMALAT1 was down-regulated, while miR-214 was elevated in SANFH tissues. MALAT1 promoted osteogenesis differentiation by sponging miR-214 to upregulate ATF4. (Source: Steroids)
Source: Steroids - November 26, 2019 Category: Drugs & Pharmacology Source Type: research

Anabolic/anticatabolic and adaptogenic effects of 24-epibrassinolide on Lupinus angustifolius: causes and consequences
Publication date: Available online 21 November 2019Source: SteroidsAuthor(s): Olga L. Kandelinskaya, Helena R. Grischenko, Vladimir A. Кhripach, Vladimir N. Zhabinskii, Lylia E. Kartizhova, Yuriy K. Shashko, Olga V. Kosmachevskaya, Elvira I. Nasybullina, Alexey F. TopunovAbstractLupinus angustifolius L. is a legume culture known as a source of valuable feed protein and the N2-fixator for improving soil fertility. However, its low ecological resistance does not allow for a stable yield of the crop. Earlier, we have shown that steroid phytohormone 24-epibrassinolide (EBR) increases the tolerance of lupine to chlorine ions b...
Source: Steroids - November 21, 2019 Category: Drugs & Pharmacology Source Type: research

Polyhydroxylated sterols from Monascus purpureus- fermented rice
Publication date: Available online 21 November 2019Source: SteroidsAuthor(s): Jin-Jie Li, Xiang-Jian Zhong, Xin Wang, Xi-Miao Yang, Jian-Yu Yue, Xuan Zhang, Jia-Chen Liu, Kai-Qi Wang, Xiao-Ya ShangAbstractUsing a cell-based cytotoxicity assay, two new polyhydroxylated sterols, 16(S),22(S)-epoxy-3β,5α,6β,20(R),23(R),25-hexahydroxy-7-ergostene and 3β,7β,8α,25-tetrahydroxy-5,22E-ergostadiene were isolated from the ethyl acetate portion of the ethanolic extract of Monascus purpureus. Their structures were elucidated by spectroscopic analysis and in comparison with those reported in the literatur...
Source: Steroids - November 21, 2019 Category: Drugs & Pharmacology Source Type: research

Microbial transformation of oral contraceptive ethisterone by Aspergillus niger and Cunninghamella blakesleeana
Publication date: February 2020Source: Steroids, Volume 154Author(s): Ambreen Aziz, Saira Bano, Atia-tul-Wahab, M. Iqbal ChoudharyAbstractEthisterone (17α-ethynyl-17β-hydroxyandrost-4-en-3-one) (1) is a synthetic steroidal estrogen. It is extensively used as an oral contraceptive. The current study involves the structural transformation of ethisterone (1) by Aspergillus niger, and Cunninghamella blakesleeana. Fermentation of 1 with C. blakesleeana afforded two new polar metabolites, 17α-ethynyl-6β,15β,17β-trihydroxyandrost-4-en-3-one, and 17α-ethynyl-7β,15β,17β-trihydrox...
Source: Steroids - November 20, 2019 Category: Drugs & Pharmacology Source Type: research

In memoriam: Virendra Bhushan Mahesh, Ph.D., D.Phil. April 25, 1932 – July 16, 2019
Publication date: Available online 13 November 2019Source: SteroidsAuthor(s): J. Charles Eldridge, Darrell W. Brann, Rajagopala Sridaran (Source: Steroids)
Source: Steroids - November 14, 2019 Category: Drugs & Pharmacology Source Type: research

Synthesis and biological evaluation of calcioic acid
Publication date: Available online 6 November 2019Source: SteroidsAuthor(s): Olivia B. Yu, Tania R. Mutchie, Elliot S. Di Milo, Leggy A. ArnoldAbstractHerein, we describe the synthesis of calcioic acid following a recently developed synthetic strategy for calcitroic acid. Several improvements to reaction conditions were made, which resulted in higher yields. The improved workup and isolation procedures are described. Furthermore, we investigated the interaction between the vitamin D receptor (VDR) and calcioic acid. Calcioic acid was able to bind VDR with a binding constant of 71 µM. In cells, calcioic acid reduced t...
Source: Steroids - November 7, 2019 Category: Drugs & Pharmacology Source Type: research

Ectopic pituitary adenoma of the TSH-secreting sphenoidal sinus with excellent response to somatostatin analogs. Theory of the embryogenesis and literature review from a clinical case
We report the case of a 52-year-old woman who was referred for primary hypothyroidism, who showed clinical signs of hyperthyroidism and had been under treatment with levothyroxine. Her exams revealed high levels of thyroid stimulating hormone (TSH), at odds with free thyroxin (FT4) and raised triiodothyronine (T3), which remained elevated after medication suspension, suggesting possible central hyperthyroidism. Sellar MRI showed normal pituitary gland, with a mass in the sphenoid sinus of 24 mm. A possible ectopic TSH secreting pituitary tumor of sphenoid sinus was hypothesized. After a single dose of a sustained-release p...
Source: Steroids - November 7, 2019 Category: Drugs & Pharmacology Source Type: research

Synthesis of Novel Hybrid Hetero-Steroids: Molecular docking Study Augmented Anti-Proliferative Properties Against Cancerous cells
In this study, a novel class of hetero-steroids were synthesized, analytical and spectral data proved the validity of the novel synthesized steroid derivatives. The cytotoxicity of the synthesized compounds 2, 5, 6, 7, 10, 11, 12, 14, 15, 17 were evaluated using human hepatocellular carcinoma cell lines (HepG2 and Huh-7) and non-small cell lung cancer (A549) cell lines. The synthesized compounds reported a remarkable gradual decrease in the cell viability of the three tested cancer cell lines. It was observed that compounds 2 and 12 had the lowest IC50s and the highest cytotoxic effects against all tested cell lines. As at...
Source: Steroids - October 31, 2019 Category: Drugs & Pharmacology Source Type: research

LncRNA-MALAT1 promotes osteogenic differentiation through regulating ATF4 by sponging miR-214 : implication of steroid-induced avascular necrosis of the femoral head
ConclusionMALAT1 was down-regulated, while miR-214 was elevated in SANFH tissues. MALAT1 promoted osteogenesis differentiation by sponging miR-214 to upregulate ATF4. (Source: Steroids)
Source: Steroids - October 31, 2019 Category: Drugs & Pharmacology Source Type: research

Estimation of the inhibiting impact of abiraterone D4A metabolite on human steroid 21-monooxygenase (CYP21A2)
Publication date: Available online 31 October 2019Source: SteroidsAuthor(s): Rami Masamrekh, Tatiana Filippova, Yaraslau Haurychenka, Kirill Shcherbakov, Alexander Veselovsky, Natallia Strushkevich, Tatsiana Shkel, Andrei Gilep, Sergey Usanov, Victoria Shumyantseva, Alexey KuzikovAbstractAbiraterone D4A metabolite, the product of 3β-hydroxysteroid dehydrogenase activity toward abiraterone, may serve as a potential antitumor agent for the treatment of prostate cancer. The main adverse effect of abiraterone is the disruption of corticosteroid biosynthesis, and the more pharmacologically active abiraterone D4A metabolite...
Source: Steroids - October 31, 2019 Category: Drugs & Pharmacology Source Type: research

New Steroidal Oxazolines, Benzoxazoles and Benzimidazoles Related to Abiraterone and Galeterone
Publication date: Available online 31 October 2019Source: SteroidsAuthor(s): Alexandra S. Latysheva, Vladimir A. Zolottsev, Alexander V. Veselovsky, Kirill A. Scherbakov, Galina E. Morozevich, Vadim S. Pokrovsky, Roman A. Novikov, Vladimir P. Timofeev, Yaroslav V. Tkachev, Alexander Y. MisharinAbstractSeven new oxazoline, benzoxazole and benzimidazole derivatives were synthesized from 3β-acetoxyandrosta-5,16-dien-17-carboxylic, 3β-acetoxyandrost-5-en-17β-carboxylic and 3β-acetoxypregn-5-en-21-oic acids. Docking to active site of human 17α-hydroxylase/17,20-lyase revealed that all oxazolines, as we...
Source: Steroids - October 31, 2019 Category: Drugs & Pharmacology Source Type: research

Antimicrobial properties of amine- and guanidine-functionalized derivatives of betulinic, ursolic and oleanolic acids: Synthesis and structure/activity evaluation
Publication date: Available online 31 October 2019Source: SteroidsAuthor(s): Anna Yu. Spivak, Rezeda R. Khalitova, Darya A. Nedopekina, Rinat R. GubaidullinAbstractA series of 34 new amine- and guanidine-functionalized derivatives of betulinic, ursolic, and oleanolic acids were synthesized and tested for their antimicrobial activity against the growth of four bacterial strains (Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus (MRSA)) and two fungal strains (Candida albicans and Cryptococcus neoforman). The obtained compounds were also tested for the cytotoxic effect against HEK29...
Source: Steroids - October 31, 2019 Category: Drugs & Pharmacology Source Type: research

Serum 25(OH)D concentration and its association with inflammation and oxidative stress in the middle-aged Indian healthy and diabetic subjects
ConclusionThis study suggest that vitamin D may have significant implications in the prevention of inflammation and oxidative stress. (Source: Steroids)
Source: Steroids - October 30, 2019 Category: Drugs & Pharmacology Source Type: research

Sarsasapogenin: A steroidal saponin from Asparagus racemosus as multi target directed ligand in Alzheimer’s Disease
Publication date: Available online 28 October 2019Source: SteroidsAuthor(s): Priya Kashyap, Kalaivani Muthusamy, Manisha Niranjan, Shweta Trikha, Suresh KumarAbstractAlzheimer’s Disease (AD) is multi-factorial disorder characterized by impaired memory and cognition deficit. AD is characterized by impaired cholinergic transmission, extracellular amyloid beta deposits , neurofibillary tangles and oxidative stress. A multi-target directed ligand (MTDL) approach is required to devise a therapeutic strategy against AD. In the present study, Asparagus racemosus aqueous extract was chosen, as it possess abundant medicinal p...
Source: Steroids - October 30, 2019 Category: Drugs & Pharmacology Source Type: research

Steroid Analysis by Ion Mobility Spectrometry
Publication date: Available online 28 October 2019Source: SteroidsAuthor(s): Alana L. Rister, Eric D. DoddsAbstractSteroids are an important biomolecule class for analysis due to their promise as biomarkers for various diseases and their abuse as performance enhancers in sports. Current analytical methods, including chromatography and nuclear magnetic resonance spectroscopy, fall short of being able to confidently analyze steroids, partly due to the large number of steroid isomers. Ion mobility spectrometry (IMS), a gas-phase ion separator, has shown potential for steroid analysis both in conjunction with liquid chromatogr...
Source: Steroids - October 30, 2019 Category: Drugs & Pharmacology Source Type: research

The impact of nandrolone decanoate abuse on experimental animal model: Hormonal and biochemical assessment
Publication date: Available online 24 October 2019Source: SteroidsAuthor(s): Neveen A. Salem, Hanan S. AlnahdiAbstractNandrolone decanoate (ND) is one of the most commonly abused anabolic androgenic steroids compounds in the world owing to its ability to improve physical performance but its abuse is associated with several adverse effects. The current study was performed to evaluate the effect of recommended and overdose of nandrolone decanoate (ND) for short and long term on the alterations of biochemical markers related to kidney, liver, adrenal, thyroid gland functions and oxidant and antioxidant activities. Sixty male ...
Source: Steroids - October 26, 2019 Category: Drugs & Pharmacology Source Type: research

Synthesis of cytotoxic urs-12-ene- and 28-norurs-12-ene- type conjugates with amino- and mercapto-1,3,4-oxadiazoles and mercapto-1,2,4-triazoles
Publication date: January 2020Source: Steroids, Volume 153Author(s): Sergey A. Popov, Marya D. Semenova, Dmitry S. Baev, Tatiana S. Frolova, Elvira E. Shults, Chengzhang Wang, Māris TurksAbstractA small library of 2-mercapto-1,3,4-oxadiazoles, 2-amino-1,3,4-oxadiazoles, and 3-mercapto-1,2,4-triazoles attached to the urs-12-ene- and 28-nor-urs-12-ene skeleton has been obtained. Ursolic acid derived hydrazides have been identified as useful starting materials for the developed synthesis. Ursolic acid hydrazide provided access to oxadiazoles attached directly to C-17 of the ursane core, but synthesis of structurally related ...
Source: Steroids - October 24, 2019 Category: Drugs & Pharmacology Source Type: research

Progesterone receptor-mediated actions and the treatment of central nervous system disorders: an up-date of the known and the challenge of the unknown
Publication date: Available online 18 October 2019Source: SteroidsAuthor(s): Susana L. González, María F. Coronel, María C. Raggio, Florencia LabombardaAbstractProgesterone has been shown to exert a wide range of remarkable protective actions in experimental models of central nervous system injury or disease. However, the intimate mechanisms involved in each of these beneficial effects are not fully depicted. In this review, we intend to give the readers a thorough revision on what is known about the participation of diverse receptors and signaling pathways in progesterone-mediated neuroprotective, pro...
Source: Steroids - October 20, 2019 Category: Drugs & Pharmacology Source Type: research

Anti-androgenic effect of 6-formylindolo[3,2-b]carbazole (FICZ) in LNCaP cells is mediated by the aryl hydrocarbon-androgen receptors cross-talk
In this study, LNCaP cells were exposed to FICZ, CH223191 and flutamide (Flu) alone or in combination in the presence and absence of testosterone. CYP1A1 enzyme activity, cell viability, cellular prostate-specific antigen (PSA) and dihydrotestosterone (DHT) production, mRNA levels of PSA, KLK2, TMPRSS2, and AR genes were measured as endpoints. A declining in the expression of androgen- responsive target genes was seen by either Flu or FICZ in the presence of testosterone. Furthermore, the forced decrease in the expression of AR target genes resulted in 41% and 31% decline in the DHT and PSA concentrations respectively. Tak...
Source: Steroids - October 20, 2019 Category: Drugs & Pharmacology Source Type: research

Crosstalk in the non-classical signal transduction of testosterone and retinol in immature rat testes
This study aimed to investigate the effects of the interaction between testosterone and retinol on the rapid responses of cultured Sertoli cells obtained from 10-day-old immature rat testes. Non-classical actions of testosterone and retinol were investigated, and the activities of L-type voltage-dependent calcium channels (L-VDCC) and voltage-dependent potassium channels (Kv) were determined by measuring 45Ca2+ influx in whole testis. Additionally, the effects of testosterone and retinol on these channels were studied in primary culture of Sertoli cells using the patch-clamp technique. 45Ca2+ influx was used to observe a d...
Source: Steroids - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Synthesis of cytotoxic urs-12-ene- and 28-nor-urs-12-ene- type conjugates with amino- and mercapto-1,3,4-oxadiazoles and mercapto-1,2,4-triazoles
Publication date: Available online 14 October 2019Source: SteroidsAuthor(s): Sergey A. Popov, Marya D. Semenova, Dmitry S. Baev, Tatiana S. Frolova, Elvira E. Shults, Chengzhang Wang, Māris TurksAbstractA small library of 2-mercapto-1,3,4-oxadiazoles, 2-amino-1,3,4-oxadiazoles and 3-mercapto-1,2,4-triazoles attached to the urs-12-ene- and 28-nor-urs-12-ene skeleton has been obtained. Ursolic acid derived hydrazides have been identified as useful starting materials for the developed synthesis. Ursolic acid hydrazide provided access to oxadiazoles attached directly to C-17 of the ursane core, but synthesis of structurally r...
Source: Steroids - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Changes of BMI, Steroid Metabolome and Psychopathology in Patients with Anorexia Nervosa During Hospitalization
Publication date: Available online 14 October 2019Source: SteroidsAuthor(s): Josef Bulant, Martin Hill, Marta Velíková, Anna Yamamotová, Pavel Martásek, Hana PapežováAbstractAnorexia nervosa (AN) is associated with various alterations including the dysfunction of the HPA axis and consequently the hypercortisolemia and deficit in sex hormones but the comprehensive evaluation of changes in circulating steroids during the hospitalization of AN patients is lacking. We investigated the effect of realimentation of women with AN during hospitalization on 45 circulating steroids, the relationshi...
Source: Steroids - October 15, 2019 Category: Drugs & Pharmacology Source Type: research

Knockdown of PTEN decreases expression of estrogen receptor β and tamoxifen sensitivity of human breast cancer cells
In this study we examined whether expression of ER β in turn might be affected by tumor suppressor PTEN, analyzed the effect of this interaction on tamoxifen response and the co-expression of both genes in human breast cancer samples. After siRNA-mediated PTEN knockdown, Western blot analysis revealed a reduction of ER β protein expression by 67.2% in MCF-7 cells and by 73.6% in T-47D cells (both p
Source: Steroids - October 9, 2019 Category: Drugs & Pharmacology Source Type: research

SUMO conjugation as regulator of the glucocorticoid receptor-FKBP51 cellular response to stress
Publication date: Available online 8 October 2019Source: SteroidsAuthor(s): Ana C. Liberman, Maia L. Budziñski, Clara Sokn, Romina P. Gobbini, Maria B. Ugo, Eduardo ArztAbstractIn order to adequately respond to stressful stimuli, glucocorticoids (GCs) target almost every tissue of the body. By exerting a negative feedback loop in the hypothalamic–pituitary–adrenal (HPA) axis GCs inhibit their own synthesis and restore homeostasis. GCs actions are mostly mediated by the GC receptor (GR), a member of the nuclear receptor superfamily. Alterations of the GR activity have been associated to different dis...
Source: Steroids - October 9, 2019 Category: Drugs & Pharmacology Source Type: research

Anti-androgenic effect of 6-formylindolo [3, 2-b] carbazole (FICZ) in LNCaP cells is mediated by the aryl hydrocarbon -androgen receptors cross-talk
In this study, an LNCaP cells were exposed to FICZ, CH223191 and flutamide (Flu) alone or in combination in the presence and absence of testosterone. CYP1A1 enzyme activity, cell viability, cellular prostate-specific antigen (PSA) and dihydrotestosterone (DHT) production, mRNA levels of PSA, KLK2, TMPRSS2, and AR genes were measured as endpoints. A declining in the expression of androgen- responsive target genes was seen by either Flu or FICZ in the presence of testosterone. Furthermore, the forced decrease in AR expression genes resulted in 41% and 31% decline in the DHT and PSA concentrations respectively. Taken together...
Source: Steroids - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Androgens are effective bronchodilators with anti-inflammatory properties: a potential alternative for asthma therapy
Publication date: Available online 3 October 2019Source: SteroidsAuthor(s): Luis M. Montaño, Edgar Flores-Soto, Bettina Sommer, Héctor Solís-Chagoyán, Mercedes PerusquíaAbstractChanges in plasma androgen levels in asthmatic men may be linked to asthma severity, seemingly acting through nongenomic and genomic effects. Nongenomic effects include rapid relaxation of carbachol or antigenic challenge pre-contracted guinea pig airway smooth muscle (ASM) in vitro: testosterone (TES) blocks L-type voltage dependent Ca2+ channels, stored operated Ca2+ channels, inositol 1,4,5-trisphosphate recepto...
Source: Steroids - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Hormonal balance and nutritional intake in elite tactical athletes
Publication date: Available online 3 October 2019Source: SteroidsAuthor(s): Andrew E. Jensen, Laura J. Arrington, Lorraine P. Turcotte, Karen R. KellyAbstractChronic exposure to multifactorial stress, such as that endured by elite military operators, may lead to overtraining syndrome and negatively impact hormonal regulation. In acute settings (
Source: Steroids - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Pharmacological Activities of Dehydroepiandrosterone: A Review
Publication date: Available online 3 October 2019Source: SteroidsAuthor(s): Poonam Sahu, Bina Gidwani, H.J. DhongadeAbstractDehydroepiandrosterone (DHEA) is a steroidal hormone secreted by Zonareticularis of the adrenal cortex with a characteristic age related pattern of secretion. These hormones are inactive precursors that are transformed into active sex steroids in peripheral target tissues. These hormones are used for the energy, vitality and the natural support of most bodily functions that involve the endocrine system. DHEA is a 19 carbon steroid hormone, is lipophilic, and can be converted to DHEAs by activity of th...
Source: Steroids - October 5, 2019 Category: Drugs & Pharmacology Source Type: research

Two new 23S,26R-hydroxylated spirostanoid saponins from the fruits of Solanum indicum var. recurvatum
Publication date: Available online 3 October 2019Source: SteroidsAuthor(s): Joseph Sakah Kaunda, Ying-Jun ZhangAbstractSolanum indicum var. recurvatum, distributed mainly in Hekou, Simao and Menghai areas of Yunnan province, China, is characterized by stellate pubescent leaves with irregular lengths and oblong, short, and downwardly curved anthers. The original species, S. indicum, has been used as an anti-inflammatory, wound-healing agent, an analgesic, and for the treatment of rhinitis, cough, and breast cancer in Chinese folk medicine. The variety was chemically studied for the first time, leading to the isolation of tw...
Source: Steroids - October 5, 2019 Category: Drugs & Pharmacology Source Type: research