Transcriptional Regulation of Zinc Transporters in Human Osteogenic Sarcoma (Saos-2) Cells to Zinc Supplementation and Zinc Depletion.

Transcriptional Regulation of Zinc Transporters in Human Osteogenic Sarcoma (Saos-2) Cells to Zinc Supplementation and Zinc Depletion. Biol Trace Elem Res. 2019 Jul 19;: Authors: Alluri K, Nair KPM, Kotturu SK, Ghosh S Abstract Bone is a passive storage organ for zinc, which contains about 30% of the total body zinc. However, during extreme zinc deficiency, only a small fraction of zinc is released in contrast to other tissues where zinc is released like monocytes or conserved, e.g., skeletal muscle. Zinc plays an important role in bone tissue remodeling. Zinc homeostasis is regulated by several zinc transporters (ZnTs) and importers (ZIPs), but their expression dynamics concerning zinc status of bone cells is not well understood. The study aimed to elucidate the effects of zinc supplementation and depletion on the transcript levels of various zinc transporters. Saos-2, a human osteoblastic cell line, was used as representative bone tissue. Zinc sulfate was used for simulating sufficient zinc status whereas TPEN, a zinc chelator, was used to simulate zinc-deficient state. Expression of various transcripts was measured by qRT-PCR. Subcellular localization of ZnT-1 was carried out by immunofluorescent microscopy, and Western Blotting was carried out to measure the expression of ZnT-1 at the protein level. Among the export transporters the transcript levels of MT, ZnT-1 showed higher levels in zinc sufficient and lower levels in TPEN tr...
Source: Biological Trace Element Research - Category: Biology Authors: Tags: Biol Trace Elem Res Source Type: research