Shotgun proteomic analysis of Bordetella parapertussis provides insights into the physiological response to iron starvation and potential new virulence determinants absent in Bordetella pertussis.

This study reports the first extensive proteome analysis of this bacterium. In an attempt to gain some insight into the infective phenotype, we evaluated the response of B. parapertussis to iron starvation, a critical stress the bacteria face during infection. Among other relevant findings, we observed that the adaptation to this condition involves significant changes in the abundance of two important virulence factors of this pathogen, namely, adenylate cyclase and the O-antigen. We further used the proteomic data to search for B. parapertussis proteins that are absent or classified as pseudogenes in the genome of Bordetella pertussis to unravel differences between both whooping cough causative agents. Among them, we identified proteins involved in stress resistance and virulence determinants that might help to explain the differences in the pathogenesis of these species and the lack of cross-protection of current acellular vaccines. Altogether, these results contribute to a better understanding of B. parapertussis biology and pathogenesis. SIGNIFICANCE: Whooping cough is a reemerging disease caused by both Bordetella pertussis and Bordetella parapertussis. Current vaccines fail to induce protection against B parapertussis and the incidence of this species has been rising over the years. The proteomic analysis of this study provided relevant insights into potential virulence determinants of this poorly-studied pathogen. It further identified proteins produced by B. parapertu...
Source: Journal of Proteomics - Category: Biochemistry Authors: Tags: J Proteomics Source Type: research

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Preterm babies were more than 20 percent less likely to have had required shots by 19 months.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Premature Babies Babies and Infants Whooping Cough Tetanus Vaccination and Immunization Hepatitis Mumps Measles Source Type: news
Authors: Polinori I, Esposito S Abstract Pertussis is an endemic highly infectious vaccine-preventable disease. The disease is a major cause of childhood morbidity and mortality. In the most recent years, the re-emergence of pertussis occurred, and many efforts were done to identify the possible causes. Certainly, more effective laboratory methods have a role in making the diagnosis easier. However, sub-optimal efficacy of available vaccines as well as their limited duration of protection could explain the resurgence of the disease. Many forms and clinical features of the disease, ranging from the most classical to...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
Condition:   Whooping Cough Interventions:   Biological: Vaccine GamLPV;   Other: Placebo Sponsor:   Gamaleya Research Institute of Epidemiology and Microbiology, Health Ministry of the Russian Federation Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Whooping Cough Interventions:   Biological: Vaccine GamLPV;   Other: Placebo Sponsor:   Gamaleya Research Institute of Epidemiology and Microbiology, Health Ministry of the Russian Federation Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Whooping Cough Interventions:   Biological: Vaccine GamLPV;   Other: Placebo Sponsor:   Gamaleya Research Institute of Epidemiology and Microbiology, Health Ministry of the Russian Federation Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Whooping Cough Interventions:   Biological: Vaccine GamLPV;   Other: Placebo Sponsor:   Gamaleya Research Institute of Epidemiology and Microbiology, Health Ministry of the Russian Federation Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Authors: Cassone A Abstract Vaccines and vaccination against pertussis (whooping cough) have had one of the longest and most complex history, with alternating splendour and public disbelief, enthusiasm and concerns, overall resulting in changes in composition and replacement of vaccines, and associated vaccination strategies, including use of different vaccines in different countries, with no apparent equals for other bacterial vaccines. Of this both frustrating and exciting venue no end has been reached. In this note, I am shortly recapitulating the history of pertussis vaccines, from the inactivated, whole-cell v...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
The objective was to analyse genomic sequences of isolates collected during the 2014 whooping cough epidemic in Tunisia, a country where whole-cell vaccines are used. Ten Tunisian isolates and four vaccine strains were sequenced and compared to 169 isolates from countries where acellular vaccines are used. Phylogenetic analysis showed that Tunisian isolates are diverse, demonstrating a multi-strain 2014 epidemic peak, and are intermixed with those circulating in other world regions, showing inter-country transmission. Consistently, Tunisian isolates have antigen variant composition observed in other world regions. No perta...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
Authors: Ring N, Abrahams JS, Bagby S, Preston A, MacArthur I Abstract The evolution of Bordetella pertussis from a common ancestor similar to Bordetella bronchiseptica has occurred through large-scale gene loss, inactivation and rearrangements, largely driven by the spread of insertion sequence element repeats throughout the genome. B. pertussis is widely considered to be monomorphic, and recent evolution of the B. pertussis genome appears to, at least in part, be driven by vaccine-based selection. Given the recent global resurgence of whooping cough despite the wide-spread use of vaccination, a more thorough unde...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
Authors: Lambert EE, Buisman AM, van Els CACM Abstract Pertussis remains endemic in vaccinated populations due to waning of vaccine-induced immunity and insufficient interruption of transmission. Correlates of long-term protection against whooping cough remain elusive but increasing evidence from experimental models indicates that the priming of particular lineages of B. pertussis (Bp) specific CD4+ T cells is essential to control bacterial load. Critical hallmarks of these protective CD4+ T cell lineages in animals are suggested to be their differentiation profile as Th1 and Th17 cells and their tissue residency. ...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
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