Validation and Retrospective Clinical Evaluation of a Quantitative 16S rRNA Gene Metagenomic Sequencing Assay for Bacterial Pathogen Detection in Body Fluids

Next-generation sequencing –based 16S rRNA gene metagenomic sequencing (16S MG) technology has tremendous potential for improving diagnosis of bacterial infections given its quantitative capability and culture-independent approach. We validated and utilized a quantitative 16S MG assay to identify and quantify bacterial spec ies in clinical samples from a wide spectrum of infections including meningitis, septic arthritis, brain abscess, intra-abdominal abscess, soft tissue abscess, and pneumonia. Twenty clinical samples were tested and 16S MG identified a total of 34 species, compared to 22 species and three descriptive findings identified by culture.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Tags: Regular article Source Type: research

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Abstract Haemophilus influenzae type b (Hib) causes various invasive infections, such as meningitis, septic arthritis, and pneumonia, especially in children under 5 years of age. Despite Hib vaccines protect Hib infection, seroepidemiological surveys of Hib infections have not been conducted systematically in Japan thus far. We analyzed 1,338 serum samples from 0-5-year-old children that were provided by the National Serum Reference Bank of the National Institute of Infectious Diseases (Tokyo, Japan). Serum levels of the anti-polyribosylribitol phosphate (PRP) IgG antibody against the Hib were determined using an ...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research
Publication date: Available online 21 June 2019Source: The Journal of Molecular DiagnosticsAuthor(s): Karissa Culbreath, Suzanne Melanson, James Gale, Justin Baker, Fan Li, Oystein Saebo, Oyvind Kommedal, Deisy Contreras, Omai B. Garner, Shangxin YangNext-generation sequencing–based 16S rRNA gene metagenomic sequencing (16S MG) technology has tremendous potential for improving diagnosis of bacterial infections given its quantitative capability and culture-independent approach. We validated and utilized a quantitative 16S MG assay to identify and quantify bacterial species in clinical samples from a wide spectrum of i...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research
CONCLUSIONS.: Pneumococcus is an important pathogen in adults, particularly in older adults, owing to its high mortality rate. PMID: 29364418 [PubMed - in process]
Source: Revista Peruana de Medicina de Experimental y Salud Publica - Category: International Medicine & Public Health Tags: Rev Peru Med Exp Salud Publica Source Type: research
Publication date: Available online 9 December 2017 Source:Infectious Disease Clinics of North America Author(s): David F. Butler, Angela L. MyersTeaser Haemophilus influenzae remains a common cause of illness in children throughout the world. Before the introduction of vaccination, H influenzae type b (Hib) disease was the leading cause of bacterial meningitis in young children and a frequent cause of pneumonia, epiglottitis, and septic arthritis. Clinicians should remain diligent in counseling parents on the dangers of Hib and provide vaccination starting at 2 months of age. The epidemiology of invasive H influenzae...
Source: Infectious Disease Clinics of North America - Category: Infectious Diseases Source Type: research
We report a case of a 73year old woman with bacteremia, endocarditis and septic arthritis due to H.influenzae serotype f. This case emphasizes the clinical features and the key elements of diagnosis and management of infections caused by non-typeable strains of H.influenzae.
Source: IDCases - Category: Infectious Diseases Source Type: research
CONCLUSION: Although invasive Hia disease can be found globally, the current epidemiological data suggest that this infection predominantly affects Indigenous communities in North America. The clinical disease of Hia and the clonal nature of the bacteria resemble that of Hib. The high incidence of invasive Hia disease in Indigenous communities, along with potential fatality and severe sequelae causing long-term disability in survivors, may support the development of a new Hia conjugate vaccine for protection against this infection similar in design to the one introduced in the 1990s to control invasive Hib disease. PM...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
We report a case of a 73year old woman with bacteremia, endocarditis and septic arthritis due to H.influenzae serotype f. This case emphasizes the clinical features and the key elements of diagnosis and management of infections caused by non-typeable strains of H.influenzae.
Source: IDCases - Category: Infectious Diseases Source Type: research
Abstract Haemophilus influenzae serotype b (Hib) was a major cause of meningitis in children until Hib conjugate vaccine was introduced into the routine infant immunization program and Hib disease in children was almost eliminated. In Alaska, northern Canada and other countries with Indigenous peoples, H. influenzae serotype a (Hia) has emerged as a significant cause of pneumonia, meningitis and septic arthritis especially in children under 24 months of age. A joint government initiative between the Public Health Agency of Canada (PHAC) and the National Research Council of Canada (NRC) was carried out to assess wh...
Source: Can Commun Dis Rep - Category: Infectious Diseases Authors: Tags: Can Commun Dis Rep Source Type: research
Atypical clinical presentations associated with group W meningococcal disease (MenW) are well-described and include pneumonia, septic arthritis, endocarditis and epiglottitis/supraglottitis. Following anecdotal reports of teenagers presenting with predominantly gastrointestinal symptoms, we undertook a case review of MenW cases in 15 to 19 year-olds diagnosed in England between July 2015 and January 2016. Of the 15 cases, seven presented with a short history of nausea, vomiting and diarrhoea; five of these seven cases died within 24 hours of presentation to hospital.
Source: Eurosurveillance - Category: Infectious Diseases Source Type: research
Abstract Atypical clinical presentations associated with group W meningococcal disease (MenW) are well-described and include pneumonia, septic arthritis, endocarditis and epiglottitis/supraglottitis. Following anecdotal reports of teenagers presenting with predominantly gastrointestinal symptoms, we undertook a case review of MenW cases in 15 to 19 year-olds diagnosed in England between July 2015 and January 2016. Of the 15 cases, seven presented with a short history of nausea, vomiting and diarrhoea; five of these seven cases died within 24 hours of presentation to hospital. PMID: 27035055 [PubMed - as supplied by publisher]
Source: Euro Surveill - Category: Infectious Diseases Authors: Tags: Euro Surveill Source Type: research
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