Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays.

Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays.
Methods Mol Biol. 2019;2017:193-203
Authors: Zhang YW, Cabezas-Wallscheid N
Abstract
Healthy hematopoietic stem cells (HSCs) are capable to self-renew and reconstitute the complete hematopoietic system. Upon aging, there is an increased incidence of blood-related diseases. Age-related phenotypes have been widely studied by bone marrow transplantation experiments, where reconstitution of the transplanted cells is a direct measure of HSC activity. In this protocol we describe a competitive bone marrow transplantation assay to functionally test young and old HSCs.
PMID: 31197778 [PubMed - in process]

Source: Mol Biol Cell - June 16, 2019 Category: Molecular Biology Authors: Zhang YW, Cabezas-Wallscheid N Tags: Methods Mol Biol Source Type: research