Opg and bcl-2 promote ameloblastoma cell tumorigenesis and predict prognosis for ameloblastoma patients

Publication date: July 2019Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, Volume 128, Issue 1Author(s): Ms. Jueyoung Kim, Ms. Jinsun Kim, Dr. Shadavlonjid Bazarsad, Prof. Sung-won Cho, Prof. Jin KimAmeloblastoma is the most frequent odontogenic epithelial tumor in the jaw. Though ameloblastoma belongs to benign odontogenic tumors, it exhibits a locally aggressive behavior with high recurrence rate. However, molecular markers predicting the recurrence have not been reported yet. The aim of this study was to find the prognostic markers in ameloblastoma. To detect apoptosis-related genes showing difference of expression level between ameloblastomas and normal oral tissues, the public database was analyzed. As results, OPG and Bcl-2 were identified as 2 most upregulated genes in ameloblastomas. To confirm public database analysis, in vitro study was conducted by use of AM-1 cell line. AM-1 cells expressed higher level of OPG and Bcl-2, compared with normal human epidermal keratinocytes (HEK). Exposing AM-1 cells to various environmental factors during culture in the 3-dimensional collagen gels were increased level of OPG and Bcl-2 than monoculture. To evaluate tumor-forming properties of AM-1 cells, subrenal capsule assay was conducted using AM-1 cells with hTERT-hNOF. As results, tumor formation were observed in 3 weeks, in which OPG and Bcl-2 expression was identified. To evaluate whether OPG and Bcl-2 regulates cell viability and apoptosis in AM-1 cell...
Source: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology - Category: ENT & OMF Source Type: research