Protective mechanism of Taxifolin for chlorpyrifos neurotoxicity in BV2 cells.

Protective mechanism of Taxifolin for chlorpyrifos neurotoxicity in BV2 cells. Neurotoxicology. 2019 May 29;: Authors: Zhang C, Zhan J, Zhao M, Dai H, Deng Y, Zhou W, Zhao L Abstract Chlorpyrifos (CPF) is an organophosphorus pesticide that can damage the central nervous system in children upon exposure. Taxifolin (Tax) exerts protective effects against neurotoxins; however, the mechanism has not yet been illustrated. The current study used BV2 cells to investigate the protective mechanism underlying the organophosphorus pesticide taxifolin on CPF-induced neurotoxicity, which might present a therapeutic potential for the prevention and treatment of the nervous system diseases in children. BV2 cells were randomly divided into 4 groups: DMSO, CPF, Tax, and Tax + CPF. The viability, morphocytology, oxidative stress, inflammatory reaction, and autophagocytosis have been studied in the cells using Western blot analysis, CCK-8 assay, enzyme-linked immunosorbent assay, and immunofluorescence to estimate the level of LC3 II. As a result, CPF was found to exert a significant toxic effect on BV2 cells that was characterized by rounded cell body, atrophic synapse, poor adhesion, cell aggregation, inflammation, oxidative reaction, and autophagy. Tax treatment has a protective effect on CPF-induced neurotoxicity via downregulation of ROS, TNF-α, IFN-γ, and p62 levels and increased LC3 II level, which in turn, improved the viability and activ...
Source: Neurotoxicology - Category: Neurology Authors: Tags: Neurotoxicology Source Type: research