Road Map for Development of Stem Cell-Based Alternative Test Methods.

Road Map for Development of Stem Cell-Based Alternative Test Methods.
Trends Mol Med. 2019 May 23;:
Authors: Sachinidis A, Albrecht W, Nell P, Cherianidou A, Hewitt NJ, Edlund K, Hengstler JG
Abstract
Much progress has been made in establishing strategies for differentiation of induced human pluripotent stem cells (hiPSCs). However, differentiated hiPSCs are not yet routinely used for prediction of toxicity. Here, limiting factors are summarised and possibilities for improvement are discussed, with a focus on hepatocytes, cardiomyocytes, tubular epithelial cells, and developmental toxicity. Moreover, we make recommendations for further fine-tuning of differentiation protocols for hiPSCs to hepatocyte-like cells by comparing individual steps of currently available protocols to the mechanisms occurring during embryonic development. A road map is proposed to facilitate test system development, including a description of the most useful performance metrics.
PMID: 31130451 [PubMed - as supplied by publisher]

Source: Trends in Molecular Medicine - May 22, 2019 Category: Molecular Biology Authors: Sachinidis A, Albrecht W, Nell P, Cherianidou A, Hewitt NJ, Edlund K, Hengstler JG Tags: Trends Mol Med Source Type: research