Bach impeller for suspension microcarrier cultures of mesenchymal stromal cells

ConclusionCulture of Wharton Jelly MSC at 1 litre with Solohill microcarriers showed 25% improved cell densities of the Bach impeller over the axial flow and paddle impellers: 4 × 10e5 cells/ml vs. 3 × 10e5 cells/ml. This was primarily due to extensive clumping of cell-microcarrier aggregates seen in the conventional impellers from day 3 onwards; whereas the laminar flow prevented aggregation up to the final day of harvesting.Significantly, when MSC were cultured on Cytodex microcarriers at 1 litre, cell yields increased dramatically to 15 × 10e5 cells/ml; viability and identity were equivalent to axial flow or spinner flask cultures. Furthermore, when MSC cultures were scaled further to 5 litre, the Bach yielded 13 × 10e5 cells/ml, and high viability. Thus, the Bach impeller is suitable for microcarrier suspension cultures of both polystyrene as well as hydrogel based microcarriers, and is a promising new impeller to add to the field of regenerative medicine for cell therapy manufacturing.
Source: Cytotherapy - Category: Cytology Source Type: research