Durable Lipopolysaccharide-Induced Potentiation of Mesenchymal Stem Cells

ConclusionIn the current study, we evaluated the feasibility of ex-vivo culture with LPS from E.coli O111:B4 as a mechanism of potentiating MSC secretion. Three independent human-derived bone marrow MSCs (BMSCs) were cultured ex-vivo in the presence of 5 µg/ml LPS. After 48 hours, conditioned medium (CM) was collected. The cells were washed and cultured for an additional 48 hours in complete medium. The CM was assayed for the presence of 42 different cytokines and growth factors using the multiplex bead platform.We found that compared to control cells not stimulated with LPS, generally ex-vivo culture with LPS significantly augmented the secretory capacity of all three primary cultures assessed. There was at least a 2-fold increase in the secretion of cytokines implicated in homing, angiogenesis and wound healing such as Eoxatin-1, GM-CSF, GRO- α and TNF-α in response to LPS stimulation. Factors critical for MSC function such as IL-6 increased 5-fold, and there was an over 100-fold increase in IL-8 secretion. Interestingly, the increase in cytokine and growth factor secretion was largely maintained up to 48 hours after the stimulation with LPS had ended. 48 hours post the termination of the ex-vivoculture with LPS, the increased fold-changes seen with IL-6, GRO- α, IL-8 and GM-CSF were preserved or only decreased minimally. Surprisingly, we noted that for some analytes such as MCP-3 and VEGF-A, the increased fold change seen 48 after the LPS stimulation had ended, was eve...
Source: Cytotherapy - Category: Cytology Source Type: research