CD34+ stromal vascular fraction was instrumental in mediating hepatic repair regarding gene expression profiles

ConclusionDonor hepatocytes (1 × 107/1mL) and SVF cells (1 × 106/0.5mL) were isolated from Tg(UBC–emGFP) rats and human adipose tissues, respectively, and were intraportally transplanted into ACLF rats. SVF cells were divided into CD34+ and CD34− groups. The surviving animals were sacrificed at 1 and 2 weeks after the transplantation. Gene expression levels, measured by quantitative real-time RT-PCR, were normalized against endogenous glyceraldehyde-3-phosphatedehydrogenase control.Histologically, prominent biliary ductular proliferation and significantly progression of fibrosis (P = 0.005) were observed in the CD34− group than in the CD34+ group. Genes directly related to the end result of fibrosis (collagen type I, MMP9, and TIMP1) showed significantly higher expression in the CD34− group than in the CD34+ group at 1 week after cell transplantation (P < 0.05). These findings were consistent with histological changes. Moreover, TGFβ1 and IRF5 had the same expression pattern with significance (P < 0.05). Fibrosis-prone histopathology in the CD34− group was associated with TGFβ1 and IRF5. On the other side, significantly higher gene expression of vascular endothelial growth factor was found in the CD34+ group compared to the CD34- group (P = 0.032), suggesting transplanted CD34+ SVF cells are key components and might lead to liver repair by modulating angiogenesis.In conclusion, hepatic gene expression in the rat model of ACLF after cytoth...
Source: Cytotherapy - Category: Cytology Source Type: research