Developing a human mesenchymal stem cell culture medium with high growth efficient, 3d culture supported, serum-free and xeno-free properties.

ConclusionThe medium on human adipose tissue-derived mesenchymal stem cells (ADSC) and Wharton's jelly mesenchymal stem cells (WJ-MSC) enables competitive expansion performance, comparing to traditional culture medium and can maintains tri-lineage mesoderm differentiation potential after fifth passages. Furthermore, most commercial serum-free media need expansive ECM coating, like fibronectin or other commercial coating materials, to support cell adhesion. Especially, AllPhase can support ADSC expansion under 3D culture condition without microcarriers. We hope that our medium can support most bioreactors and cell industrial production for MSC clinical applications.
Source: Cytotherapy - Category: Cytology Source Type: research