Salmonella Typhi, Paratyphi A, Enteritidis and Typhimurium core proteomes reveal differentially expressed proteins linked to the cell surface and pathogenicity

Conclusions/SignificanceOur comparative proteome analysis indicated differences in the expression of surface proteins betweenSalmonella Typhi and Paratyphi A, and in pathogenesis-related proteins between Salmonella Typhimurium and Enteritidis. Our findings may guide future development of novel diagnostics and vaccines, as well as understanding of disease progression.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research

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Abstract Increasing awareness of Salmonella Paratyphi A's contribution to enteric fever episodes throughout Asia has led to the development of new S. Paratyphi A vaccines. Assays are needed to measure functional antibodies elicited by the new vaccine candidates to assess their immunogenicity and potential protective capacities. Serum bactericidal assay (SBA) is the method of choice to measure functional antibody titers against various bacterial pathogens, but it is rarely been used for large dataset and clinical samples because it is time consuming and labor-intensive. Recently we developed a high-throughput lumin...
Source: Journal of Immunological Methods - Category: Allergy & Immunology Authors: Tags: J Immunol Methods Source Type: research
In this study, cytotoxicity, protective efficacy, and immune responses of the host were analyzed. Our previous data had shown a significance decrease in virulence for the crp and sipB mutants compared with a wild-type strain. The current study confirmed this finding in HeLa cells and showed that the crp mutant was significantly less cytotoxic (P
Source: Canadian Journal of Veterinary Research - Category: Veterinary Research Authors: Tags: Can J Vet Res Source Type: research
In this study, we separately cloned genes necessary for the biosynthesis of the S. flexneri serotype 2a and 3a O-antigens, which have been shown previously to provide broad cross-protection to multiple, disease-predominant S. flexneri serotypes. The cloned S. flexneri 2a rfb operon along with, bgt and gtrII, encoded on the SfII bacteriophage were sufficient in Ty21a to express the heterologous S. flexneri 2a O-antigen containing the 3,4 antigenic determinants. Further, this rfb operon along with gtrA, gtrB and gtrX encoded on the Sfx bacteriophage and oac encoded on the Sf6 bacteriophage were sufficient to express S. flexn...
Source: Clinical and Vaccine Immunology - Category: Allergy & Immunology Authors: Tags: Clin Vaccine Immunol Source Type: research
by Ros ângela Salerno-Gonçalves, Hervé Tettelin, David Lou, Stephanie Steiner, Tasmia Rezwanul, Qin Guo, William D. Picking, Vishvanath Nene, Marcelo B. SzteinSalmonella enterica serovar Typhi (S. Typhi), the causative agent of the typhoid fever, is a pathogen of great public health importance. Typhoid vaccines have the potential to be cost-effective measures towards combating this disease, yet the antigens triggering host protective immune responses are largely unknown. Given the key role of cellular-mediated immunity inS. Typhi protection, it is crucial to identifyS. Typhi proteins involved in T-cell ...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
In this study, an S. Typhi ghost was developed and its capacity as a vaccine candidate against typhoid fever was assessed. METHODOLOGY: An asd+ plasmid pJHL187 harbouring a ghost cassette comprising the PhiX 174 Elysis gene tightly controlled under the convergent promotor system was transformed into an asd gene-deleted mutant S.Typhi strain (STG). The eltB gene encoding the E. coli heat-labile enterotoxin (LTB) protein was subcloned into a foreign antigen delivery cassette of pJHL187 to increase mucosal immunity. RESULTS: The stringent repression and expression of the lethal E lysis gene in the system allowed sta...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
by Zoe A. Dyson, Duy Pham Thanh, Ladaporn Bodhidatta, Carl Jeffries Mason, Apichai Srijan, Maia A. Rabaa, Phat Voong Vinh, Tuyen Ha Thanh, Guy E. Thwaites, Stephen Baker, Kathryn E. Holt Vaccines againstSalmonella Typhi, the causative agent of typhoid fever, are commonly used by travellers, however, there are few examples of national immunization programs in endemic areas. There is therefore a paucity of data on the impact of typhoid immunization programs on localised populations ofS. Typhi. Here we have used whole genome sequencing (WGS) to characterise 44 historical bacterial isolates collected before and after a nation...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Conclusions and SignificanceSalmonella T3SS SipD is a promising antigen for the development of a protectiveSalmonella vaccine, and could be developed for vaccination in tropical endemic areas to control infant mortality.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
In this report, we tested the hypothesis that probiotic organisms could compete for the preferred nutrient sources of Salmonella and thus prevent or treat infection. To this end, we added the fra locus, which encodes a utilization pathway for the Salmonella-specific nutrient source fructose-asparagine (F-Asn), to the probiotic bacterium Escherichia coli Nissle 1917 (Nissle) to increase its ability to compete with Salmonella in mouse models. We also tested a metabolically competent, but avirulent, Salmonella enterica serovar Typhimurium mutant for its ability to compete with wild-type Salmonella. The modified Nissle strain ...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Microbial Immunity and Vaccines Source Type: research
Publication date: August 2016 Source:Microbial Pathogenesis, Volume 97 Author(s): Mi Seon Jang, Sushant Sahastrabuddhe, Cheol-Heui Yun, Seung Hyun Han, Jae Seung Yang Typhoid fever, mainly caused by Salmonella enterica serovar Typhi (S. Typhi), is a life-threatening disease, mostly in developing countries. Enzyme-linked immunosorbent assay (ELISA) is widely used to quantify antibodies against S. Typhi in serum but does not provide information about functional antibody titers. Although the serum bactericidal assay (SBA) using an agar plate is often used to measure functional antibody titers against various bacterial ...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Salmonella enterica causes systemic diseases (typhoid and paratyphoid fever), nontyphoidal septicemia (NTS), and gastroenteritis in humans and other animals worldwide. An important but underrecognized emerging infectious disease problem in sub-Saharan Africa is NTS in children and immunocompromised adults. A current goal is to identify Salmonella mutants that are not pathogenic in the absence of key components of the immune system such as might be found in immunocompromised hosts. Such attenuated strains have the potential to be used as live vaccines. We have used transposon-directed insertion site sequencing (TraDIS) to s...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Microbial Immunity and Vaccines Source Type: research
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