Spinal CCL1/CCR8 regulates phosphorylation of GluA1-containing AMPA receptor in postoperative pain after tibial fracture and orthopedic surgery in mice

This study examined whether spinal CCL1 is associated with fracture-associated postoperative pain via AMPA receptor.We herein discovered that the tibial fracture with orthopedic surgery initiated and maintained chronic postoperative pain along with spinal up-regulation of CCL1/CCR8 expression and phosphorylation of GluA1-containing AMPA receptor. Central CCL1/CCR8 inhibition impaired mechanical and cold allodynia, and phosphorylated GluA1-containing AMPA receptor in the spinal dorsal horn. Intrathecal injection of GluA1-containing AMPA receptor antagonist NASPM alleviated fracture-related postoperative pain. Also, exogenous CCL1 delivery facilitated acute pain behaviors and spinal phosphorylation of GluA1-containing AMPA receptor in naïve mice, reversing by co-application of NASPM. Our current results indicated that spinal CCL1/CCR8-mediated GluA1-containing AMPA receptor activation is vital in the pathogenesis of fracture associated postoperative pain in mice.
Source: Neuroscience Research - Category: Neuroscience Source Type: research

Related Links:

ConclusionsMusculoskeletal problems are the main occupational health problems experienced by the study group. New findings include a low but important prevalence of ultraviolet radiation–associated conditions. One in 3 guides have experienced significant psychological trauma, and one quarter of these had symptoms of PTSD.
Source: Wilderness and Environmental Medicine - Category: Environmental Health Source Type: research
Condition:   Chronic Low-back Pain Interventions:   Behavioral: Mindfulness-based dance/movement therapy (M-DMT);   Behavioral: Chronic pain social support group Sponsors:   Drexel University;   National Center for Complementary and Integrative Health (NCCIH);   Stony Brook University Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Neuropathic Pain Intervention:   Procedure: Lumbar Puncture Sponsor:   Guy's and St Thomas' NHS Foundation Trust Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Chronic Low-back Pain Interventions:   Behavioral: Mindfulness-based dance/movement therapy (M-DMT);   Behavioral: Chronic pain social support group Sponsors:   Drexel University;   National Center for Complementary and Integrative Health (NCCIH);   Stony Brook University Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Neuropathic Pain Intervention:   Procedure: Lumbar Puncture Sponsor:   Guy's and St Thomas' NHS Foundation Trust Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Chronic Low-back Pain Interventions:   Behavioral: Mindfulness-based dance/movement therapy (M-DMT);   Behavioral: Chronic pain social support group Sponsors:   Drexel University;   National Center for Complementary and Integrative Health (NCCIH);   Stony Brook University Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Neuropathic Pain Intervention:   Procedure: Lumbar Puncture Sponsor:   Guy's and St Thomas' NHS Foundation Trust Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Chronic Low-back Pain Interventions:   Behavioral: Mindfulness-based dance/movement therapy (M-DMT);   Behavioral: Chronic pain social support group Sponsors:   Drexel University;   National Center for Complementary and Integrative Health (NCCIH);   Stony Brook University Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Condition:   Neuropathic Pain Intervention:   Procedure: Lumbar Puncture Sponsor:   Guy's and St Thomas' NHS Foundation Trust Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
In this study, we explored the proposed PNM‐CAN mechanism in SCI + NP and AB cohorts following centrally‐directed neuromodulation to assess if the PNM and CAN are capable of being differentially modulated.Materials and MethodsCentral neuromodulation was administered via breathing ‐controlled electrical stimulation (BreEStim), previously evidenced to operate at the PNM. To quantify CAN activity, conventional heart rate variability (HRV) recordings were used to gather time and frequency domain parameters of autonomic modulation. SCI + NP (n = 10) and AB (n = 13) cohorts received null and act...
Source: Neuromodulation: Technology at the Neural Interface - Category: Biotechnology Authors: Tags: Clinical Research Source Type: research
More News: Back Pain | Brain | Chronic Pain | Neurology | Neuroscience | Neurosurgery | Orthopaedics | Pain | Study