Biosynthesis of β-d-glucan‑gold nanoparticles, cytotoxicity and oxidative stress in mouse splenocytes.

This study reports biosynthesis of gold-nanoparticles (AuNPs) by using β-d-glucans isolated from the yeast Yarrowia lypolitica D1. β-d-glucans serve as reducing and stabilizing mediators that induce the formation of AuNPs on the outer surface of the own β-d-glucan. The systems were physicochemically characterized by ultraviolet visible (UV-Vis) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), and dynamic light scattering (DLS) analyses. The results revealed the generation of AuNPs with quasi-spherical shape or large one dimension (1D) gold-nanostructures (AuNSs) depending on the HAuCl4 concentration. A cytotoxic study was assessed in mouse splenocytes. Contrary to that expected, important cytotoxicity was found in all β-d-gluc+AuNPs systems by oxidative stress increase. This study discusses the cytotoxic mechanism, suggesting that the resulting β-d-gluc+AuNPs systems may not be candidates for the formulation of immunostimulants or nanocarriers for biomedical applications.
PMID: 31082425 [PubMed - as supplied by publisher]

Source: International Journal of Biological Macromolecules - May 9, 2019 Category: Biochemistry Authors: Hernández-Adame L, Angulo C, Delgado K, Schiavone M, Castex M, Palestino G, Betancourt-Mendiola L, Reyes-Becerril M Tags: Int J Biol Macromol Source Type: research