Structural analysis of a replication protein encoded by a plasmid isolated from a multiple sclerosis patient

In this study, a Rep WH1 domain encoded on a BMMF (MSBI1.176) isolated from a multiple sclerosis human brain sample was determined to 1.53   Å resolution using X-ray crystallography. The overall structure of the MSBI1.176 WH1 domain was remarkably similar to other Rep structures, despite having a low (28%) amino-acid sequence identity. The MSBI1.176 WH1 domain contained elements common to other Reps, including five α -helices, five β -strands and a hydrophobic pocket. These new findings suggest that the MSBI1.176 Rep might have comparable roles and functions to other known Reps of different origins.
Source: Acta Crystallographica Section D - Category: Biochemistry Authors: Tags: bovine meat and milk factors episomal circular DNA trans-acting replication initiator protein multiple sclerosis X-ray crystal structure research papers Source Type: research