Crustacean hyperglycemic hormone (CHH) affects hemocyte intracellular signaling pathways to regulate exocytosis and immune response in white shrimp Litopenaeus vannamei

Publication date: Available online 26 April 2019Source: PeptidesAuthor(s): Xu Lijun, Pan Luqing, Xin Zhang, Wei CunAbstractRecombinant Litopenaeus vannamei CHH (rLvCHH) was obtained from a bacterial expression system and the intracellular signaling pathways involved in exocytosis and immune response after rLvCHH injection (0.2 and 2 μg/shrimp) was investigated in this study. The results showed that CHH contents increased 51.4%-110.2% (0.2 μg/shrimp) and 65.0%-211.3% (2 μg/shrimp) of the control level. And the contents of three biogenic amines in hemolymph presented a similar variation pattern after rLvCHH injection, but reached the highest level at different time points. Furthermore, the mRNA expression levels of membrane-bound guanylyl cyclase (mGC) (1.20-1.93 fold) and biogenic amine receptors, including type 2 dopamine receptor (DA2R) (0.72-0.89 fold), α2 adrenergic receptor (α2-AR) (0.72-0.91 fold) and 5-HT7 receptor (5-HT7R) (1.37-3.49 fold) in hemocytes were changed consistently with their ligands. In addition, the second messenger and protein kinases shared a similar trend and reached the maximum at the same time respectively. The expression levels of nuclear transcription factor (cAMP response element-binding protein, CREB) and exocytosis-related proteins transcripts were basically overexpressed after rLvCHH stimulation, which reached the peaks at 1 h or 3 h. Eventually, the phenoloxidase (PO) activity (37.4%-158.5%) and antibacterial activity (31.8%-12...
Source: Peptides - Category: Biochemistry Source Type: research