487 Dynamics of IL-1R signaling in innate and adaptive immunity against a Staphylococcus aureus skin infection in mice

IL-1 β/IL-1R/MyD88-signaling is critical in mediating neutrophil recruitment and host defense against S. aureus skin infections but the cells that respond to IL-1β to trigger the protective IL-1R-dependent immune response are unclear. To evaluate the relative contribution of different IL-1R-expressing cell types against a community-acquired methicillin-resistant S. aureus (CA-MRSA) skin infection, a CA-MRSA strain (USA300 LAC::lux; 3x107 CFU) was inoculated intradermally into cre/lox mice with specific deletion of IL-1R in which IL-1R-floxed mice (IL-1Rfl/fl) were bred to mice with cre-specific e xpression in keratinocytes (K14-cre), all T cells (Lck-cre), all CD4+/CD8+ T cells (CD4-cre), dendritic cells (CD11c-cre) and neutrophils (S100A8-cre).
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Innate Immunity, Microbiology, and Microbiome Source Type: research