Gradual and Asynchronous Increase of Temperature Influence on Insulin Endo- and Exocytosis in Isolated Vertebrata Hepatocytes.

Gradual and Asynchronous Increase of Temperature Influence on Insulin Endo- and Exocytosis in Isolated Vertebrata Hepatocytes. Curr Protein Pept Sci. 2019 Mar 24;: Authors: Kolychev AP, Terpilovskii MA Abstract Insulin internalization and intracellular insulin/receptor complex (IRC) processing provide with both the hormonal signal transduction elongation into cell and the dissociated complex parts utilization. The patterns of temperature influence on the insulin mechanism of action (IAM), in spite of a general opinion on temperature factor significance, have not been yet revealed. The results of the experimental studies previously carried out on four vertebrate classes isolated hepatocytes (lamprey, frogs, chickens, rats) and the data analysis have revealed the temperature factor particular effects on IAM separate stages in a family of vertebrates and clarified the number of important circumstances. 1- Some essential differentiations of insulin endo and exocytosis activity in poikilothermic and endothermic vertebrate hepatocytes have been found out. 2 - Besides, some significant changes of temperature influence on IAM in a cell have been revealed: a) At the 125 I-insulin internalization high level in cyclostomes (lamprey) hepatocytes the total absence of the process receptibility to temperature has been detected; b) The 125 I-insulin internalization receptibility to temperature at amphibians has been defined but only in the narrow ra...
Source: Current Protein and Peptide Science - Category: Biochemistry Authors: Tags: Curr Protein Pept Sci Source Type: research