Isolation and purification of fucoidan fraction in Turbinaria ornata from the Maldives; inflammation inhibitory potential under LPS stimulated conditions in in-vitro and in-vivo models.

Isolation and purification of fucoidan fraction in Turbinaria ornata from the Maldives; inflammation inhibitory potential under LPS stimulated conditions in in-vitro and in-vivo models. Int J Biol Macromol. 2019 Mar 18;: Authors: Jayawardena TU, Fernando IPS, Lee WW, Sanjeewa KKA, Kim HS, Lee DS, Jeon YJ Abstract Fucoidan, referred to as fucose containing sulfated polysaccharides (FCSP), is a polymer from brown algae cell wall that is reported to exhibit potential anti-inflammatory activity. In the present study, the fucoidans are extracted from Turbinaria ornata (TO) from the Maldives. The method involves enzyme assisted extraction and is modified in order to improve the effectiveness and purity of final product. Purified fucoidan fraction was identified as F10, and its chemical properties were verified via FTIR, 1H NMR and monosaccharide analysis. Selected inflammatory mediators were studied to evaluate the anti-inflammatory potential using RAW 264.7 macrophages. F10 successfully inhibited NO production (IC50 = 30.83 ± 1.02 μg mL-1). F10 dose-dependently down-regulated iNOS, COX-2, and pro-inflammatory cytokines including PGE2 levels. The in vivo experiments were assisted by zebrafish embryo model. This exhibited reduction in ROS, NO expression levels. To our knowledge, this is the first report to illustrate potential anti-inflammatory activity of FCSPs' extracted from the brown algae T. ornata. Concisely, the results...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research