Long-term self-renewal of naïve neural stem cells in a defined condition

Publication date: Available online 9 March 2019Source: Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchAuthor(s): Yongming Wu, Xi Chen, Guangjun Xi, Xingliang Zhou, Suyue Pan, Qi-Long YingAbstractDuring embryonic development, neural stem cells (NSCs) emerge as early as the neural plate stage and give rise to the nervous system. Early-stage NSCs express Sry-related-HMG box-1 (Sox1) and are biased towards neuronal differentiation. However, long-term maintenance of early-stage NSCs in vitro remains a challenge. Here, we report development of a defined culture condition for the long-term maintenance of Sox1-positive early-stage mouse NSCs. The proliferative ability of these Sox1-positive NSCs was confirmed by clonal propagation. Compared to the NSCs cultured using the traditional culture condition, the long-term self-renewing Sox1-positive NSCs efficiently differentiate into neurons and exhibit an identity representative of the anterior and midbrain regions. These early-stage Sox1-positive NSCs could also be switched to late-stage NSCs by being cultured with bFGF/EGF, which can then differentiate into astrocytes and oligodendrocytes. The long-term self-renewing Sox1-positive NSCs were defined as naïve NSCs, based on their high neuronal differentiation capacity and anterior regional identity. This culture condition provides a robust platform for further dissection of the NSC self-renewal mechanism and promotes potential applications of NSCs for cell-based therapy...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - Category: Molecular Biology Source Type: research

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Source: Cell - Category: Cytology Source Type: research
Publication date: 13 June 2019Source: Cell, Volume 177, Issue 7Author(s): Diana CrowAn “off-the-shelf” cell therapy derived from induced pluripotent stem cells (iPSCs) has entered clinical trials in the United States. Other companies are following suit, harnessing iPSCs’ self-renewal ability to manufacture cell therapies that don’t require customization for each patient. But some experts aren’t sure such therapies are a good idea.
Source: Cell - Category: Cytology Source Type: research
Publication date: Available online 14 June 2019Source: NeuroscienceAuthor(s): Christopher A. BlackwoodAbstractRadial glial maintenance is essential for the proper development of the cortex. It is known that the evolutionarily conserved Notch signaling pathway is required for maintaining the pool of radial glial stem cells although the mechanisms involved are not entirely understood. Here, we study the Notch ligand, Jagged1, in the mouse ventricular zone at a late stage of embryonic development. We use a conditional loss of function allele to show that Jagged1 is required for maintaining radial glial cells and when absent, ...
Source: Neuroscience - Category: Neuroscience Source Type: research
Publication date: Available online 14 June 2019Source: NeuroscienceAuthor(s): Olena Lykhmus, Olena Kalashnyk, Lyudmyla Koval, Larysa Voytenko, Kateryna Uspenska, Serhiy Komisarenko, Olena Deryabina, Nadia Shuvalova, Vitalii Kordium, Alina Ustymenko, Vitalii Kyryk, Maryna SkokAbstractNicotinic acetylcholine receptors of α7 subtype (α7 nAChRs) are involved in regulating cognition, inflammation and cell survival. Neuroinflammation is accompanied by the decrease of α7 nAChRs in the brain and impairment of memory. We show here that α7 −/− mice possess pro-inflammatory phenotype and demon...
Source: Neuroscience - Category: Neuroscience Source Type: research
Contributors : Yuelin Song ; Patrick R van den Berg ; Rudolf Jaenisch ; Stefan SemrauSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusVariable levels of DNA methylation have been reported at tissue-specific differential methylation regions (DMRs) overlapping enhancers, including super-enhancers (SEs) associated with key cell identity genes, but the mechanisms responsible for this intriguing behavior are not well understood. We used allele-specific reporters at the endogenous Sox2 and miR290 super-enhancers (SE) in embryonic stem cells and found that the allelic DNA methylation state is...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Contributors : Yuelin Song ; Bingbing Yuan ; Rudolf JaenischSeries Type : Methylation profiling by high throughput sequencingOrganism : Mus musculusVariable levels of DNA methylation have been reported at tissue-specific differential methylation regions (DMRs) overlapping enhancers, including super-enhancers (SEs) associated with key cell identity genes, but the mechanisms responsible for this intriguing behavior are not well understood. We used allele-specific reporters at the endogenous Sox2 and miR290 super-enhancers (SE) in embryonic stem cells and found that the allelic DNA methylation state is dynamically switching, ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Methylation profiling by high throughput sequencing Mus musculus Source Type: research
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Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research
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Source: Expert Opinion on Investigational Drugs - Category: Drugs & Pharmacology Tags: Expert Opin Investig Drugs Source Type: research
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