Horizon scanning for novel and emerging in vitro mammalian cell mutagenicity test systems

Publication date: Available online 21 February 2019Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Stephen J. Evans, Bhaskar Gollapudi, Martha M. Moore, Shareen H. DoakAbstractThe induction of gene mutation within a DNA sequence can result in an adverse impact, altering or preventing gene function. Therefore, in vitro evaluation of mutagenicity is an essential component of the toxicological screening process. A variety of mutagen screening tools are routinely used in genetic toxicology, which are based on selected reporter genes. These assays are however typically labour intensive and impractical for high throughput screening. Considering this, the IWGT (International Workshops on Genotoxicity Testing) sub-group on Novel & Emerging In Vitro Mammalian Cell Mutagenicity Test Systems undertook a literature search to identify new approaches for mutation detection. This review therefore focused on identifying new approaches for mutation detection that have the potential for use as a future genotoxicity screening tool. A comprehensive literature review identified genome-wide loss-of-function screening tools, next generation sequencing (NGS) mutation characterisation and fluorescence-based mutation detection methods as having significant promise as an emerging in vitro mammalian cell mutagenicity test system. Each of the technologies considered was assessed for its capacity to report on a wide array of heritable mutagenic changes, necessary to co...

Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - February 22, 2019 Category: Genetics & Stem Cells Source Type: research