A multidirectional investigation of stem bark extracts of four African plants: HPLC-MS/MS profiling and biological potentials

Publication date: Available online 19 February 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Kouadio Bene, Kouadio Ibrahime Sinan, Gokhan Zengin, Alina Diuzheva, József Jekő, Zoltán Cziáky, Muhammad Zakariyyah Aumeeruddy, Jianbo Xiao, Mohamad Fawzi MahomoodallyAbstractThe practice of traditional medicine, especially herbal medicine, is still prevalent across the African continent. Yet, their in-depth pharmacological and chemical exploitation by the scientific community remain a necessity. The aim of the present study was to investigate into the phenolic components, antioxidant, and enzyme-inhibitory activities of three solvent extracts (ethyl acetate, methanol, and water) of the stem bark of four African plant species (Senna siamea, Distemonanthus benthamianus, Harrisonia abyssinica, and Pycnanthus angolensis). It was found that D. benthamianus followed by P. angolensis, displayed the highest DPPH and ABTS scavenging, ferric and cupric reducing, and total antioxidant capacity in the phosphomolybdenum assay. A similar result was observed for AChE, BChE, and tyrosinase inhibition. The two plants also showed comparable α-amylase inhibitory effect. On the other hand, H. abyssinica showed high metal chelating and α-glucosidase inhibition. Among the solvents used, the methanol extract seemed to be the most bioactive. In addition, TPC was highest in D. benthamianus (135.33-192.29 mg GAE/g) while P. angolensis was richest in TFC (7.68-12.48 mg RE/g...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research