Construction of a novel far-red fluorescence light-up probe for visualizing intracellular peroxynitrite.

Construction of a novel far-red fluorescence light-up probe for visualizing intracellular peroxynitrite.
Talanta. 2019 May 15;197:431-435
Authors: Qu W, Niu C, Zhang X, Chen W, Yu F, Liu H, Zhang X, Wang S
Abstract
Peroxynitrite (ONOO-) is an important reactive oxygen species (ROS), which can react with a variety of biologically active species and cause many diseases, such as cancer, neurodegenerative disorders. Herein, we develop a novel far-red fluorescent probe DCM-KA, which is equipping with α-ketoamide moiety caged DCM-NH2. The probe exhibits fluorescence off-on response to ONOO- over other biological interfering analytes by ONOO--induced α-ketoamide deprotection reaction. More importantly, the probe is biocompatible and has been successfully utilized to visualize endogenous ONOO- production in macrophage cell line J774A.1.
PMID: 30771958 [PubMed - in process]

Source: Talanta - February 19, 2019 Category: Chemistry Authors: Qu W, Niu C, Zhang X, Chen W, Yu F, Liu H, Zhang X, Wang S Tags: Talanta Source Type: research