Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells.

Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells. J Biol Regul Homeost Agents. 2019 Feb 14;33(1) Authors: Brązert M, Iżycki D, Kranc W, Borowiec B, Popis M, Celichowski P, Ożegowska K, Jankowski M, Jeseta M, Pawelczyk L, Bręborowicz A, Rachoń D, Nowicki M, Kempisty B Abstract The ovarian granulosa cells (GCs) that form the structure of follicle undergo substantial modification during the various stages of human folliculogenesis. These modifications include morphological changes, accompanied by differential expression of genes, encoding proteins which are mainly involved in cell growth, proliferation and differentiation. Recent data bring a new insight into the aspects of GCs' stem-like specificity and plasticity, enabling their prolonged proliferation and differentiation into other cell types. This manuscript focuses attention on emerging alterations during GC cell cycle - a series of biochemical and biophysical changes within the cell. Human GCs were collected from follicles of women set to undergo intracytoplasmic sperm injection procedure, as a part of remnant follicular fluid. The cells were primarily cultured for 30 days. Throughout this time, we observed the prominent change in cell morphology from epithelial-like to fibroblast-like, suggesting differentiation to other cell types. Additionally, at days 1, 7, 15 and 30, the RNA was isolated for molecular assays. Using Affymetrix® Huma...
Source: Journal of Biological Regulators and Homeostatic Agents - Category: Biomedical Science Tags: J Biol Regul Homeost Agents Source Type: research