De novo annotation of the transcriptome of the Northern Wheatear (Oenanthe oenanthe).

De novo annotation of the transcriptome of the Northern Wheatear (Oenanthe oenanthe). PeerJ. 2018;6:e5860 Authors: Frias-Soler RC, Villarín Pildaín L, Hotz-Wagenblatt A, Kolibius J, Bairlein F, Wink M Abstract We have sequenced a partial transcriptome of the Northern Wheatear (Oenanthe oenanthe), a species with one of the longest migrations on Earth. The transcriptome was constructed de novo using RNA-Seq sequence data from the pooled mRNA of six different tissues: brain, muscle, intestine, liver, adipose tissue and skin. The samples came from nine captive-bred wheatears collected at three different stages of the endogenous autumn migratory period: (1) lean birds prior the onset of migration, (2) during the fattening stage and (3) individuals at their migratory body mass plateau, when they have almost doubled their lean body mass. The sample structure used to build up the transcriptome of the Northern Wheatears concerning tissue composition and time guarantees the future survey of the regulatory genes involved in the development of the migratory phenotype. Through the pre-migratory period, birds accomplish outstanding physical and behavioural changes that involve all organ systems. Nevertheless, the molecular mechanisms through which birds synchronize and control hyperphagia, fattening, restlessness increase, immunity boosting and tuning the muscles for such endurance flight are still largely unknown. The use of RNA-Seq has emerged...
Source: Genomics Proteomics ... - Category: Genetics & Stem Cells Authors: Tags: PeerJ Source Type: research