Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy.

Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy.
Methods Mol Biol. 2019;1900:167-179
Authors: Mittmann S, Arrieta M, Ramsay L, Waugh R, Colas I
Abstract
Recombination (crossover) drives the release of genetic diversity in plant breeding programs. However, in barley, recombination is skewed toward the telomeric ends of its seven chromosomes, restricting the re-assortment of about 30% of the genes located in the centromeric regions of its large 5.1 Gb genome. A better understanding of meiosis and recombination could provide ways of modulating crossover distribution and frequency in barley as well as in other grasses, including wheat. While most research on recombination has been carried out in the model plant Arabidopsis thaliana, recent studies in barley (Hordeum Vulgare) have provided new insights into the control of crossing over in large genome species. A major achievement in these studies has been the use of cytological procedures to follow meiotic events. This protocol provides detailed practical steps required to perform immunostaining of barley meiocytes (pollen mother cells) for confocal or structured illumination microscopy.
PMID: 30460565 [PubMed - in process]

Source: Mol Biol Cell - November 23, 2018 Category: Molecular Biology Authors: Mittmann S, Arrieta M, Ramsay L, Waugh R, Colas I Tags: Methods Mol Biol Source Type: research