Molecular analysis of peripheral lung adenocarcinoma in brush cytology obtained by endobronchial ultrasound plus fluoroscopy-guided

The aim of our study was to evaluate the feasibility of molecular analysis of EGFR and KRAS from brushing specimens obtained by radial endobronchial ultrasound plus fluoroscopy-guided (R-EBUS) in patients with peripheral pulmonary adenocarcinoma. We prospectively included patients who underwent bronchoscopy guided by fluoroscopy and R-EBUS for the study of peripheral pulmonary lesions (PPL) between July 2015 and July 2017. Samples (brushing specimens) were taken in cytological extensions for diagnosis and brush conserved in RPMI culture medium for molecular study. In the cases diagnosed of adenocarcinoma, DNA was isolated for mutational analysis of EGFR.108 patients with PPL underwent bronchoscopy, of which 91 were neoplasms. Bronchoscopy guided by fluoroscopy and R-EBUS were performed in 70 patients with definitive diagnosis of malignancy. Finally, 30 patients with adenocarcinoma were included in the study. In 100%, the molecular study for KRAS and EGFR was viable with brushing specimens. Of the 30 cases, in 16 (53.3%) KRAS or EGFR mutation were detected: 10 KRAS (34.4%) and 6 (20%) EGFR and in 2 patients different EGFR mutations were detected. When comparing with histological samples, a correlation of 86.6% was detected and only in 2 wild-type patients in brushing specimens an EGFR mutation was detected in the histological sample.The present study demonstrated that brushing specimens conserved in RPMI medium and obtained by R-EBUS-guided bronchoscopy plus fluoroscopy are va...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Lung Cancer Source Type: research