TROAP regulates prostate cancer progression via the WNT3/survivin signalling pathways.

TROAP regulates prostate cancer progression via the WNT3/survivin signalling pathways. Oncol Rep. 2018 Nov 09;: Authors: Ye J, Chu C, Chen M, Shi Z, Gan S, Qu F, Pan X, Yang Q, Tian Y, Wang L, Yang W, Cui X Abstract Prostate cancer (PCa) is one of the most commonly diagnosed malignancies, and 90% of advanced prostate cancer patients relapse after therapy. Trophinin associated protein (TROAP) is essential for centrosome integrity and proper bipolar organisation of spindle assembly during mitosis and plays an essential role in proliferation. We found that TROAP expression correlates with patient survival and speculated that it may be involved in PCa progression. The Oncomine database tool (http://www.oncomine.org) was used to analyse TROAP mRNA expression from microarray data, and patient survival analysis for target genes was performed using the PROGgeneV2 Database (http://watson.compbio.iupui.edu). Gene interference with lentivirus was used to silence TROAP expression in PCa cells and knockdown efficiency was detected by qRT-PCR and western blot analysis. Cell viability, colony formation, cell cycle and apoptosis were then assessed to determine the function of TROAP in PCa cells. Markers of cell cycle and apoptosis were tested by western blotting. The correlation between WNT3 or survivin expression and TROAP transcripts in prostate cancer tissues was analysed using GEPIA (http://gepia.cancer-pku.cn) and validated by western blotting....
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research