Impaired DNA double ‐strand breaks repair by kinesin family member 4A inhibition renders human H1299 non‐small‐cell lung cancer cells sensitive to cisplatin

Cisplatin treatment stimulates the expression of kinesin family member 4A (KIF4A) protein in human non ‐small‐cell lung cancer (NSCLC) cells. KIF4A knockdown suppresses the formation of BRCA2 and Rad51 foci and limits the further increase in poly(ADP‐ribose) polymerase 1 activity, thereby enhancing cisplatin‐induced cell cycle arrest in S and G2/M phases and cytotoxicity in human NSCLC cells. KIF4A functions as a novel modulator of cisplatin sensitivity in human NSCLC cells via multiple mechanisms. AbstractPatients with non ‐small‐cell lung cancer (NSCLC) are routinely treated with the platinum‐based chemotherapeutics such as cisplatin. The drug exerts anticancer effects via multiple mechanisms, including DNA double‐strand breaks (DSBs). Enhanced DNA DSB repair capacity would be associated with innate or acquir ed drug resistance. However, despite strong evidence for the role of the chromokinesin kinesin family member 4A (KIF4A) in DSB repair, the relationship between the chromokinesin and cisplatin sensitivity of human NSCLC cells remains unknown. Furthermore, little is known regarding the effect of targe ting KIF4A on the function of DSB repair‐related proteins in these cells. In the current study, we demonstrated that cisplatin treatment stimulated the expression of KIF4A protein in human NSCLC cells. Depletion of KIF4A by small interfering RNA significantly enhanced cisplatin‐induced cell cycl e arrest in S and G2/M phases and cytotoxicity in human NSC...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research