Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150glued

In this study, we found that cell proliferation was reduced by CQ treatment in time and dose ‐dependent manners. No obvious cell death was detected; however, what was observed instead was G0/G1 arrest during which primary cilium started to grow in the presence of CQ. Pharmacological inhibition of primary cilium formation led to a reduction of cell viability suggesting that CQ‐induced pr imary cilium protected cells from death. In addition to cell growth, with the CQ treatment the retina pigmented epithelium (RPE) cells less flattened with the spindle‐like protrusion. When checking the microtubule networks, the microtubule nucleation activity was disrupted in the presence of CQ. T he level of p150glued, the largest subunit of dynactin, was reduced in CQ ‐treated RPE1 cells, and depletion of p150glued resulted in a phenotype reminiscent of CQ ‐treated cells. Thus, CQ treatment reduced the expression of p150glued, leading to reduced S phase entry and defective microtubule nucleation.
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research