Chromosomal scan of single sperm cells by combining fluorescence-activated cell sorting and next-generation sequencing

ConclusionsWe optimized FACS protocol for simple and efficient isolation of single human sperm cells that subsequently enabled a successful genome-wide chromosome profiling and identification of segmental aneuploidies from these individual cells, following NGS analysis. This approach may be useful for analyzing semen samples of infertile men or chromosomal aberration carriers to facilitate the reproductive risk assessment.
Source: Journal of Assisted Reproduction and Genetics - Category: Reproduction Medicine Source Type: research