Hepatotoxic effects of Euphol-rich fractions from Euphorbia bivonae—Relevance to cytotoxic and anti-tumor activities

Publication date: Available online 1 November 2018Source: PathophysiologyAuthor(s): Khaled Athmouni, Abdelfattah El Feki, Habib AyadiAbstractThese studies were designed to evaluate the preliminary oral toxicity profile of the crude ethanolic aerial part extract of E. bivonae in the Male albino Wistar rats and its active chemical constituents. The 24-h LD50 was determined using probit analysis method. The single dose LD50was found to be 2568.64 mg/kg bw when administrated orally in mice. Additionally, the Wistar rats were used to evaluate the subchronic toxicity of E. bivonae ethanolic extract. The serum biomarkers, lipid peroxidation and antioxidants status in liver and histopathological analysis were investigated in normal and treated groups. Subchronic toxicity studies in rats with oral doses of 50, 150, 350 and 500 mg/kg body weight showed significant increase in alanine aminotransferase, aspartate aminotransferase and total bilirubin levels. In addition, the administration of this extract significantly (p < 0.05) decreased superoxide dismutase, catalase and glutathione peroxidase and an increment in lipid peroxidation and protein carbonyls. Finally, we suggest that the three compounds of E. bivonae extract (sitosterol, euphol and lupeol) are the mainly responsible of this toxicity.

Source: Pathophysiology - November 2, 2018 Category: Pathology Source Type: research