Effects of six compounds with different chemical structures on melanogenesis

Publication date: October 2018Source: Chinese Journal of Natural Medicines, Volume 16, Issue 10Author(s): Rakotomalala Manda Heriniaina, Jing DONG, Praveen Kumar Kalavagunta, Hua-Li WU, Dong-Sheng YAN, Jing SHANGAbstractSeveral chemical compounds can restore pigmentation in vitiligo through mechanisms that vary according to disease etiology. In the present study, we investigated the melanogenic activity of six structurally distinct compounds, namely, scopoletin, kaempferol, chrysin, vitamin D3, piperine, and 6-benzylaminopurine. We determined their effectiveness, toxicity, and mechanism of action for stimulating pigmentation in B16F10 melanoma cells and in a zebrafish model. The melanogenic activity of 6-benzylaminopurine, the compound identified as the most potent, was further verified by measuring green fluorescent protein concentration in tyrp1 a: eGFP (tyrosinase-related protein 1) zebrafish and mitfa: eGFP (microphthalmia associated transcription factor) zebrafish and antioxidative activity. All the tested compounds were found to enhance melanogenesis responses both in vivo and in vitro at their respective optimal concentration by increasing melanin content and expression of TYR and MITF. 6-Benzyamino-purine showed the strongest re-pigmentation action at a concentration of 20 μmol·L−1in vivo and 100 μmol·L−1in vitro, and up-regulated the strong fluorescence expression of green fluorescent protein in tyrp1a: eGFP and mitfa: eGFP zebrafish in vitro. However, its re...
Source: Chinese Journal of Natural Medicines - Category: Complementary Medicine Source Type: research