GSE113382 RisA-dependent small regulatory RNA is involved in glutamate uptake in the human pathogen Bordetella pertussis

Contributors : Kristina Keidel ; Branislav Vecerek ; David HotSeries Type : Expression profiling by arrayOrganism : Bordetella pertussisBordetella pertussis is the causative agent of human whooping cough (pertussis), a highly contagious respiratory disease. Recently we have shown that the RNA chaperone Hfq plays a significant role in virulence of Bordetella pertussis. This suggested that Hfq-dependent small regulatory RNAs could be involved in modulation of gene expression in this re-emerging pathogen. High through-put RNA sequencing methods combined with biocomputational tools revealed hundreds of putative non-coding RNAs including a potential sRNA candidate upstream of the uncharacterized BP2735 gene s2013 sRNA. Further characterization of this candidate, for which we suggest the name s2013 for reasons expound later, showed that its aAbundance of this sRNA is strongly decreased in absence of RNA chaperone Hfq and expression is modulated by the activities of the two-component regulatory system BvgAS and another response regulator RisA. Whereas s2013 levels were elevated under modulatory conditions or in the absence of bvg genes, the deletion of the risA gene completely abolished s2013 expression. Profiling of the Δs2013 mutant in the ΔbvgA genetic background identified BP3831 gene encoding an ABC transporter periplasmic amino acid-binding protein as an exclusive significantly modulated target. Our data indicate that s2013 sRNA base-pairs with BP3831 mRNA around the Shin...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Bordetella pertussis Source Type: research