The detection of age-, gender-, and region-specific changes in mouse brain tocopherol levels via the application of different validated HPLC methods.

The detection of age-, gender-, and region-specific changes in mouse brain tocopherol levels via the application of different validated HPLC methods. Neurochem Res. 2018 Sep 07;: Authors: Nánási N, Veres G, Cseh EK, Szentirmai M, Martos D, Sümegi E, Hadady L, Klivényi P, Vécsei L, Zádori D Abstract The aging process clearly increases the demand for antioxidant protection, especially in the brain, involving that provided by α-tocopherol (αT). However, little is known about the age-related changes in brain αT levels and the influencing effect of gender on it, in human or murine samples as well. Accordingly, the aim of the current study was to detect age-, gender- and region-specific changes in αT concentrations in mouse brain tissue and to assess the influencing effect of plasma αT levels on it. Female and male C57BL/6 mice at the ages of 6, 16 and 66 weeks (n = 9 in each group) were applied. αT levels were determined with high performance liquid chromatography (HPLC) from the striatum, cortex, hippocampus, cerebellum, brainstem and from plasma samples. A detailed validation process was carried out for the applied HPLC method as well. The results demonstrated that brain αT levels significantly increased in the striatum, cortex, and hippocampus with aging in both genders, but in a more pronounced way in females with an increasing magnitude of this difference. In case of the cerebellum, a moderate elevation could be dete...
Source: Neurochemical Research - Category: Neuroscience Authors: Tags: Neurochem Res Source Type: research